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The Journal of Neuroscience, June 7, 2006, 26(23):6259-6268; doi:10.1523/JNEUROSCI.0481-06.2006

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Cellular/Molecular
Insulin-Like Growth Factor-1 Modulation of CaV1.3 Calcium Channels Depends on Ca²⁺ Release from IP₃-Sensitive Stores and Calcium/Calmodulin Kinase II Phosphorylation of the 1 Subunit EF Hand

Lei Gao, ^* Leslie A. C. Blair, ^* Gregory D. Salinas, Leigh A. Needleman, and John Marshall

Department of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, Rhode Island 02912

Correspondence should be addressed to Dr. Leslie A. C. Blair, Department of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, G-B489, Providence, RI 02912. Email: Leslie_Blair{at}Brown.edu

In neurons, L-type calcium channels (CaV1.2 and CaV1.3) regulate an extensive range of functions. However, the roles of CaV1.3-containing L channels, which are physiologically and pharmacologically distinct from the better understood CaV1.2 channels, are only beginning to be determined. We find that CaV1.3 channels are modulated by the insulin-like growth factor-1/receptor tyrosine kinase (IGF-1/RTK) through a signaling pathway that involves phospholipase C, calcium release from IP₃-sensitive internal stores, and calcium/calmodulin kinase II. In addition, we find that the IGF-1-induced modulation requires phosphorylation of a specific serine residue, S1486, in the EF hand motif of the CaV1.3 subunit. This modulation alters CaV1.3 activity, causing a left shift in the current–voltage relationship and strongly potentiating peak currents at hyperpolarized membrane potentials. We also find that CaV1.3 channels and their RTK-dependent potentiation contribute to the regulation of the survival-promoting transcription factor cAMP response element-binding protein (CREB): in both cortical and hippocampal neurons, depolarization and IGF-1 rapidly increase phospho-CREB levels in a manner that requires CaV1.3 activity and the S1486 phosphorylation site to achieve a full effect. Although the full effects of CaV1.3 channels remain to be determined, their preferential localization to dendritic shafts and spine heads coupled with their ability to activate at relatively hyperpolarized and even subthreshold potentials suggests that CaV1.3 activity may subserve different cellular functions from CaV1.2 and, in particular, may be important in transducing signals initiated by excitatory neurotransmission.

Key words: CaV1.3; CaMKII; IP₃; IGF-1; Shank; CREB

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Received Oct. 10, 2005; revised April 5, 2006; accepted April 5, 2006.

Correspondence should be addressed to Dr. Leslie A. C. Blair, Department of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, G-B489, Providence, RI 02912. Email: Leslie_Blair{at}Brown.edu

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