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The Journal of Neuroscience, August 9, 2006, 26(32):8398-8408; doi:10.1523/JNEUROSCI.0618-06.2006

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Development/Plasticity/Repair
Calcium Activation of the LMO4 Transcription Complex and Its Role in the Patterning of Thalamocortical Connections

Amir H. Kashani,¹ Zilong Qiu,² Linda Jurata,³ Soo-Kyung Lee,³ Samuel Pfaff,³ Sandra Goebbels,⁴ Klaus-Armin Nave,⁴ and Anirvan Ghosh^1,2

¹Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, ²Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093-0366, ³Gene Expression Laboratory, The Salk Institute, La Jolla, California 92037, and ⁴Department of Neurogenetics, Max Planck Institute of Experimental Medicine, D-37075 Goettingen, Germany

Correspondence should be addressed to Anirvan Ghosh, Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093-0366. Email: aghosh{at}ucsd.edu

Lasting changes in neuronal connectivity require calcium-dependent gene expression. Here we report the identification of LIM domain-only 4 (LMO4) as a mediator of calcium-dependent transcription in cortical neurons. Calcium influx via voltage-sensitive calcium channels and NMDA receptors contributes to synaptically induced LMO4-mediated transactivation. LMO4-mediated transcription is dependent on signaling via calcium/calmodulin-dependent protein (CaM) kinase IV and microtubule-associated protein (MAP) kinase downstream of synaptic stimulation. Coimmunoprecipitation experiments indicate that LMO4 can form a complex with cAMP response element-binding protein (CREB) and can interact with cofactor of LIM homeodomain protein 1 (CLIM1) and CLIM2. To evaluate the role of LMO4 in vivo, we examined the consequences of conditional loss of lmo4 in the forebrain, using the Cre-Lox gene-targeting strategy. The organization of the barrel field in somatosensory cortex is disrupted in mice in which lmo4 is deleted conditionally in the cortex. Specifically, in contrast to controls, thalamocortical afferents in conditional lmo4 null mice fail to segregate into distinct barrel-specific domains. These observations identify LMO4 as a calcium-dependent transactivator that plays a key role in patterning thalamocortical connections during development.

Key words: LMO4; calcium; transcription; barrel cortex; development; cortex

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Received Nov. 9, 2004; revised June 14, 2006; accepted July 5, 2006.

Correspondence should be addressed to Anirvan Ghosh, Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093-0366. Email: aghosh{at}ucsd.edu

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