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The Journal of Neuroscience, August 16, 2006, 26(33):8622-8632; doi:10.1523/JNEUROSCI.3967-05.2006

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Neurobiology of Disease
Multiprotein Complexes of the Survival of Motor Neuron Protein SMN with Gemins Traffic to Neuronal Processes and Growth Cones of Motor Neurons

Honglai Zhang,^2 * Lei Xing,^1 * Wilfried Rossoll,¹ Hynek Wichterle,³ Robert H. Singer,² and Gary J. Bassell¹

¹Department of Cell Biology and Center for Neurodegenerative Disease, Emory University, Atlanta, Georgia 30322, ²Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, and ³Department of Pathology, Columbia University College of Medicine, New York, New York 10032

Correspondence should be addressed to Dr. Gary Bassell, Department of Cell Biology, Emory University, 615 Michael Street, Atlanta, GA 30322. Email: gbassel{at}emory.edu

Spinal muscular atrophy (SMA), a progressive neurodegenerative disease affecting motor neurons, is caused by mutations or deletions of the SMN1 gene encoding the survival of motor neuron (SMN) protein. In immortalized non-neuronal cell lines, SMN has been shown to form a ribonucleoprotein (RNP) complex with Gemin proteins, which is essential for the assembly of small nuclear RNPs (snRNPs). An additional function of SMN in neurons has been hypothesized to facilitate assembly of localized messenger RNP complexes. We have shown that SMN is localized in granules that are actively transported into neuronal processes and growth cones. In cultured motor neurons, SMN granules colocalized with ribonucleoprotein Gemin proteins but not spliceosomal Sm proteins needed for snRNP assembly. Quantitative analysis of endogenous protein colocalization in growth cones after three-dimensional reconstructions revealed a statistically nonrandom association of SMN with Gemin2 (40%) and Gemin3 (48%). SMN and Gemin containing granules distributed to both axons and dendrites of differentiated motor neurons. A direct interaction between SMN and Gemin2 within single granules was indicated by fluorescence resonance energy transfer analysis of fluorescently tagged and overexpressed proteins. High-speed dual-channel imaging of live neurons depicted the rapid and bidirectional transport of the SMN–Gemin complex. The N terminus of SMN was required for the recruitment of Gemin2 into cytoplasmic granules and enhanced Gemin2 stability. These findings provide new insight into the molecular composition of distinct SMN multiprotein complexes in neurons and motivation to investigate deficiencies of localized RNPs in SMA.

Key words: survival of motor neuron protein; SMN; spinal muscular atrophy; SMA; RNA granules; mRNA localization; growth cone; motor neuron disease

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Received Sept. 19, 2005; revised July 10, 2006; accepted July 10, 2006.

Correspondence should be addressed to Dr. Gary Bassell, Department of Cell Biology, Emory University, 615 Michael Street, Atlanta, GA 30322. Email: gbassel{at}emory.edu

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