QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, November 1, 2006, 26(44):11432-11436; doi:10.1523/JNEUROSCI.1660-06.2006

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by Price, G. D. Articles by Trussell, L. O. Search for Related Content PubMed PubMed Citation Articles by Price, G. D. Articles by Trussell, L. O.

 Previous Article  |  Next Article 

Brief Communications
Estimate of the Chloride Concentration in a Central Glutamatergic Terminal: A Gramicidin Perforated-Patch Study on the Calyx of Held

Gareth D. Price and Laurence O. Trussell

Oregon Hearing Research Center, Vollum Institute, Oregon Health and Science University, Portland, Oregon 97239

Correspondence should be addressed to Laurence O. Trussell, Oregon Hearing Research Center, Vollum Institute, Oregon Health and Science University, Mail Code L-335A, 3181 Southwest Sam Jackson Park Road, Portland, OR 97239. Email: trussell{at}ohsu.edu

The function of presynaptic terminals is regulated by intracellular Cl^–, the levels of which modify vesicular endocytosis and transmitter refilling and mediate the effects of presynaptic ligand-gated Cl^– channels. Nevertheless, the concentration of Cl^– in a central nerve terminal is unknown, and it is unclear whether terminals can regulate Cl^– independently of the soma. Using perforated-patch recording in a mammalian synapse, we found that terminals accumulate Cl^– up to 21 mM, between four and five times higher than in their parent cell bodies. Changing [Cl^–] did not alter vesicular glutamate content in intact terminals, unlike in vitro experiments. Thus, glutamatergic terminals maintain an elevated Cl^– concentration without compromising synaptic transmission.

Key words: chloride; perforated-patch; calyx of Held; auditory; glycinergic; KCC2

---

Received April 18, 2006; revised Sept. 20, 2006; accepted Sept. 22, 2006.

Correspondence should be addressed to Laurence O. Trussell, Oregon Hearing Research Center, Vollum Institute, Oregon Health and Science University, Mail Code L-335A, 3181 Southwest Sam Jackson Park Road, Portland, OR 97239. Email: trussell{at}ohsu.edu

This article has been cited by other articles:

				Z.-Q. Tang, H. Gao, and Y. Lu Control of a Depolarizing GABAergic Input in an Auditory Coincidence Detection Circuit J Neurophysiol, September 1, 2009; 102(3): 1672 - 1683. [Abstract] [Full Text] [PDF]

				P. Long, A. Mercer, R. Begum, G. J. Stephens, T. S. Sihra, and J. N. Jovanovic Nerve Terminal GABAA Receptors Activate Ca2+/Calmodulin-dependent Signaling to Inhibit Voltage-gated Ca2+ Influx and Glutamate Release J. Biol. Chem., March 27, 2009; 284(13): 8726 - 8737. [Abstract] [Full Text] [PDF]

				S. Khirug, J. Yamada, R. Afzalov, J. Voipio, L. Khiroug, and K. Kaila GABAergic Depolarization of the Axon Initial Segment in Cortical Principal Neurons Is Caused by the Na-K-2Cl Cotransporter NKCC1 J. Neurosci., April 30, 2008; 28(18): 4635 - 4639. [Abstract] [Full Text] [PDF]

				L. Li, J. Bischofberger, and P. Jonas Differential Gating and Recruitment of P/Q-, N-, and R-Type Ca2+ Channels in Hippocampal Mossy Fiber Boutons J. Neurosci., December 5, 2007; 27(49): 13420 - 13429. [Abstract] [Full Text] [PDF]

				R. Renden and H. von Gersdorff Synaptic Vesicle Endocytosis at a CNS Nerve Terminal: Faster Kinetics at Physiological Temperatures and Increased Endocytotic Capacity During Maturation J Neurophysiol, December 1, 2007; 98(6): 3349 - 3359. [Abstract] [Full Text] [PDF]

				F. F. Trigo, M. Chat, and A. Marty Enhancement of GABA Release through Endogenous Activation of Axonal GABAA Receptors in Juvenile Cerebellum J. Neurosci., November 14, 2007; 27(46): 12452 - 12463. [Abstract] [Full Text] [PDF]

				W. T. Nickell, N. K. Kleene, and S. J. Kleene Mechanisms of neuronal chloride accumulation in intact mouse olfactory epithelium J. Physiol., September 15, 2007; 583(3): 1005 - 1020. [Abstract] [Full Text] [PDF]

				I. Milenkovic, M. Witte, R. Turecek, M. Heinrich, T. Reinert, and R. Rubsamen Development of Chloride-Mediated Inhibition in Neurons of the Anteroventral Cochlear Nucleus of Gerbil (Meriones unguiculatus) J Neurophysiol, September 1, 2007; 98(3): 1634 - 1644. [Abstract] [Full Text] [PDF]

				B. M. Stell, P. Rostaing, A. Triller, and A. Marty Activation of Presynaptic GABAA Receptors Induces Glutamate Release from Parallel Fiber Synapses J. Neurosci., August 22, 2007; 27(34): 9022 - 9031. [Abstract] [Full Text] [PDF]

				H. Alle and J. R. P. Geiger GABAergic Spill-Over Transmission onto Hippocampal Mossy Fiber Boutons J. Neurosci., January 24, 2007; 27(4): 942 - 950. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help