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The Journal of Neuroscience, November 22, 2006, 26(47):12127-12136; doi:10.1523/JNEUROSCI.3158-06.2006

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Cellular/Molecular
Regional Interaction of Endoplasmic Reticulum Ca²⁺ Signals between Soma and Dendrites through Rapid Luminal Ca²⁺ Diffusion

Yu Mi Choi, Shin Hye Kim, Sungkwon Chung, Dae Yong Uhm, and Myoung Kyu Park

Department of Physiology, Sungkyunkwan University School of Medicine and Center For Molecular Medicine, Samsung Biomedical Research Institute, 300 Chunchun-dong, Jangan-ku, Suwon, 440-746, Korea

Correspondence should be addressed to Assoc. Prof. Myoung Kyu Park, Department of Physiology, Sungkyunkwan University School of Medicine, 300 Chunchun-dong, Jangan-ku, Suwon 440-746, Korea. Email: mkpark{at}med.skku.ac.kr

The endoplasmic reticulum (ER) Ca²⁺ store plays a key role in integration and conveyance of Ca²⁺ signals in highly polarized neurons. The interconnected ER network in neurons generates Ca²⁺ signals in local domains, but the regional interaction is unclear. Here, we show that continuous or repetitive applications of caffeine produced robust Ca²⁺ release from the ER Ca²⁺ store in dendritic areas without severe store depletion, but that similar stimuli applied to soma caused rapid store depletion in acutely isolated midbrain dopamine neurons. Partial emptying of the ER Ca²⁺ store within a dendrite caused a similar level of store depletion in unstimulated dendrites, as well as in soma. Photobleaching and local stimulation experiments revealed that Ca²⁺ and the dye trapped within the ER diffused rapidly from the soma to dendrites up to 90 µm, which we could resolve, suggesting that the ER network acts as a functional tunnel for rapid Ca²⁺ transport. These data imply that the ER in soma acts as a Ca²⁺ reservoir supplying Ca²⁺ to the dendritic store, and that the dendritic store, hence, is able to respond to Ca²⁺-mobilizing input signals endurably.

Key words: Ca²⁺ signal; endoplasmic reticulum; intracellular Ca²⁺ store; neuron; dopaminergic neuron; Ca²⁺ concentration in the endoplasmic reticulum; photobleaching

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Received July 24, 2006; revised Oct. 12, 2006; accepted Oct. 14, 2006.

Correspondence should be addressed to Assoc. Prof. Myoung Kyu Park, Department of Physiology, Sungkyunkwan University School of Medicine, 300 Chunchun-dong, Jangan-ku, Suwon 440-746, Korea. Email: mkpark{at}med.skku.ac.kr

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