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The Journal of Neuroscience, December 20, 2006, 26(51):13328-13337; doi:10.1523/JNEUROSCI.2858-06.2006

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Development/Plasticity/Repair
Netrin/DCC Signaling Controls Contralateral Dendrites of Octavolateralis Efferent Neurons

Arminda Suli,¹ Nathan Mortimer,³ Iain Shepherd,³ and Chi-Bin Chien^1,2

¹Department of Neurobiology and Anatomy and ²Brain Institute, University of Utah, Salt Lake City, Utah 84132, and ³Department of Biology, Emory University, Atlanta, Georgia 30322

Correspondence should be addressed to Chi-Bin Chien, Department of Neurobiology and Anatomy, 401 MREB, University of Utah Medical Center, 20 North 1900 East, Salt Lake City, UT 84132-3401. Email: chi-bin.chien{at}neuro.utah.edu

The guidance molecule Netrin and its receptor DCC (deleted in colorectal cancer) attract commissural axons toward the midline en route to their final destination. To test whether these molecules can also guide dendrites, we studied the contralateral dendrites of zebrafish octavolateralis efferent (OLe) neurons, which are unusual in that they navigate toward and cross the midline. We found that, at the time of dendrite outgrowth, OLe neurons express dcc, and the hindbrain midline expresses netrin1. Knocking down dcc or netrin1 function by injecting antisense morpholino oligonucleotides prevented OLe contralateral dendrites from crossing the midline, showing that dcc and netrin1 are necessary for dendrite guidance or formation. Furthermore, by transplanting cells from dcc morphants into wild-type embryos and vice versa, we demonstrated that dcc acts cell autonomously in OLe dendrites. This work is the first evidence that Netrin/DCC signaling acts in dendrites in a vertebrate system.

Key words: Netrin; DCC; dendritic guidance; zebrafish; hindbrain; octavolateralis

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Received July 5, 2006; revised Nov. 15, 2006; accepted Nov. 16, 2006.

Correspondence should be addressed to Chi-Bin Chien, Department of Neurobiology and Anatomy, 401 MREB, University of Utah Medical Center, 20 North 1900 East, Salt Lake City, UT 84132-3401. Email: chi-bin.chien{at}neuro.utah.edu

This article has been cited by other articles:

				E. Kastenhuber, U. Kern, J. L. Bonkowsky, C.-B. Chien, W. Driever, and J. Schweitzer Netrin-DCC, Robo-Slit, and Heparan Sulfate Proteoglycans Coordinate Lateral Positioning of Longitudinal Dopaminergic Diencephalospinal Axons J. Neurosci., July 15, 2009; 29(28): 8914 - 8926. [Abstract] [Full Text] [PDF]

				M. M. Corty, B. J. Matthews, and W. B. Grueber Molecules and mechanisms of dendrite development in Drosophila Development, April 1, 2009; 136(7): 1049 - 1061. [Abstract] [Full Text] [PDF]

				T. Di Meglio, K. T. Nguyen-Ba-Charvet, M. Tessier-Lavigne, C. Sotelo, and A. Chedotal Molecular Mechanisms Controlling Midline Crossing by Precerebellar Neurons J. Neurosci., June 18, 2008; 28(25): 6285 - 6294. [Abstract] [Full Text] [PDF]

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