QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, February 8, 2006, 26(6):1803-1812; doi:10.1523/JNEUROSCI.3611-05.2006

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (20) Citing Articles via Google Scholar Google Scholar Articles by Sasaki, M. Articles by Kocsis, J. D. Search for Related Content PubMed PubMed Citation Articles by Sasaki, M. Articles by Kocsis, J. D.

 Previous Article  |  Next Article 

Development/Plasticity/Repair
Molecular Reconstruction of Nodes of Ranvier after Remyelination by Transplanted Olfactory Ensheathing Cells in the Demyelinated Spinal Cord

Masanori Sasaki, Joel A. Black, Karen L. Lankford, Hajime A. Tokuno, Stephen G. Waxman, and Jeffery D. Kocsis

Department of Neurology and Center for Neuroscience and Regeneration Research, Yale University School of Medicine, New Haven, Connecticut 06510, and Rehabilitation Research Center, Veterans Affairs Connecticut Healthcare System, West Haven, Connecticut 06516

Correspondence should be addressed to Dr. Jeffery D. Kocsis, Department of Neurology, Yale University School of Medicine, Neuroscience Research Center (127A), Veterans Affairs Connecticut Healthcare System, West Haven, CT 06516. Email: jeffery.kocsis{at}yale.edu

Myelin-forming glial cells transplanted into the demyelinated spinal cord can form compact myelin and improve conduction properties. However, little is known of the expression and organization of voltage-gated ion channels in the remyelinated central axons or whether the exogenous cells provide appropriate signaling for the maturation of nodes of Ranvier. Here, we transplanted olfactory ensheathing cells from green fluorescent protein (GFP)-expressing donor rats [GFP-olfactory ensheathing cells (OECs)] into a region of spinal cord demyelination and found extensive remyelination, which included the development of mature nodal, paranodal, and juxtaparanodal domains, as assessed by ultrastructural, immunocytochemical, and electrophysiological analyses. In remyelinated axons, Na_v1.6 was clustered at nodes, whereas K_v1.2 was aggregated in juxtaparanodal regions, recapitulating the distribution of these channels within mature nodes of uninjured axons. Moreover, the recruitment of Na_v and K_v channels to specific membrane domains at remyelinated nodes persisted for at least 8 weeks after GFP-OEC transplantation. In vivo electrophysiological recordings demonstrated enhanced conduction along the GFP-OEC-remyelinated axons. These findings indicate that, in addition to forming myelin, engrafted GFP-OECs provide an environment that supports the development and maturation of nodes of Ranvier and the restoration of impulse conduction in central demyelinated axons.

Key words: axon; spinal cord injury; potassium channels; olfactory bulb; sodium channel; remyelination; transplantation; demyelination

---

Received Aug. 25, 2005; revised Dec. 16, 2005; accepted Dec. 29, 2005.

Correspondence should be addressed to Dr. Jeffery D. Kocsis, Department of Neurology, Yale University School of Medicine, Neuroscience Research Center (127A), Veterans Affairs Connecticut Healthcare System, West Haven, CT 06516. Email: jeffery.kocsis{at}yale.edu

This article has been cited by other articles:

				M. Dubois-Dalcq, A. Williams, C. Stadelmann, B. Stankoff, B. Zalc, and C. Lubetzki From fish to man: understanding endogenous remyelination in central nervous system demyelinating diseases Brain, July 1, 2008; 131(7): 1686 - 1700. [Abstract] [Full Text] [PDF]

				J. D. Kocsis and S. G. Waxman Schwann cells and their precursors for repair of central nervous system myelin Brain, August 1, 2007; 130(8): 1978 - 1980. [Full Text] [PDF]

				A. Toft, D. T. Scott, S. C. Barnett, and J. S. Riddell Electrophysiological evidence that olfactory cell transplants improve function after spinal cord injury Brain, April 1, 2007; 130(4): 970 - 984. [Abstract] [Full Text] [PDF]

				E. Eftekharpour, S. Karimi-Abdolrezaee, J. Wang, H. El Beheiry, C. Morshead, and M. G. Fehlings Myelination of Congenitally Dysmyelinated Spinal Cord Axons by Adult Neural Precursor Cells Results in Formation of Nodes of Ranvier and Improved Axonal Conduction J. Neurosci., March 28, 2007; 27(13): 3416 - 3428. [Abstract] [Full Text] [PDF]

				Y. Yang, Y. Ogawa, K. L. Hedstrom, and M. N. Rasband {beta}IV spectrin is recruited to axon initial segments and nodes of Ranvier by ankyrinG J. Cell Biol., February 12, 2007; 176(4): 509 - 519. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help