QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, February 15, 2006, 26(7):1913-1922; doi:10.1523/JNEUROSCI.3571-05.2006

This Article Full Text Full Text (PDF) Supplemental data Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Tsai, H.-H. Articles by Miller, R. H. Search for Related Content PubMed PubMed Citation Articles by Tsai, H.-H. Articles by Miller, R. H.

 Previous Article  |  Next Article 

Development/Plasticity/Repair
Netrin-1 Is Required for the Normal Development of Spinal Cord Oligodendrocytes

Hui-Hsin Tsai,¹ Wendy B. Macklin,² and Robert H. Miller¹

¹Department of Neurosciences, Case Western Reserve University, Cleveland, Ohio 44106, and ²Cleveland Clinic Foundation, Department of Neurosciences, Lerner Research Center, Cleveland, Ohio 44195

Correspondence should be addressed to Robert H. Miller, Department of Neurosciences, Case Western Reserve University, School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106. Email: rhm3{at}case.edu

Successful CNS myelination is dependent on the correct localization of oligodendrocytes and their interactions with adjacent axons. In the spinal cord, oligodendrocyte precursors originate at the ventral midline and subsequently migrate to the white matter where they mature. In vitro studies suggest this dispersal is mediated by the guidance molecule netrin-1. Here, we show that in the spinal cord of netrin-1 mutant mice, oligodendrocyte precursors failed to disperse from the ventral midline as a consequence of a lack of polarization and directional migration. The lack of netrin-1 also resulted in an overall reduction of oligodendrocyte lineage cells that was independent of the failure of initial dispersal. Oligodendrocyte precursors injected into presumptive white matter underwent extensive radial migration and expansion in wild-type but not netrin-1 mutant hosts. These data indicate that netrin-1 is crucial for both the initial dispersal of spinal cord oligodendrocyte precursors and their subsequent development in the presumptive white matter.

Key words: oligodendrocyte precursor; migration; netrin-1; cytoarchitecture; time-lapse imaging; slice cultures

---

Received Aug. 23, 2005; revised Dec. 25, 2005; accepted Dec. 29, 2005.

Correspondence should be addressed to Robert H. Miller, Department of Neurosciences, Case Western Reserve University, School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106. Email: rhm3{at}case.edu

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help