 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
The Journal of Neuroscience, March 1, 2006, 26(9):2531-2543; doi:10.1523/JNEUROSCI.1234-05.2006
Previous Article | Next Article
Cellular/Molecular
Functional Analysis of Circadian Pacemaker Neurons in Drosophila melanogaster
Dirk Rieger,1 Orie Thomas Shafer,2 Kenji Tomioka,3 andCharlotte Helfrich-Förster1 1University of Regensburg, Institute of Zoology, 93040 Regensburg, Germany, 2Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110, and 3Department of Biology, Faculty of Science, Okayama University, Okayama 700-8530, Japan
Correspondence should be addressed to Charlotte Helfrich-Förster, University of Regensburg, Institute of Zoology, Universitaetsstraße 31, 93040 Regensburg, Germany. Email: charlotte.foerster{at}biologie.uni-regensburg./.de
The molecular mechanisms of circadian rhythms are well known, but how multiple clocks within one organism generate a structured rhythmic output remains a mystery. Many animals show bimodal activity rhythms with morning (M) and evening (E) activity bouts. One long-standing model assumes that two mutually coupled oscillators underlie these bouts and show different sensitivities to light. Three groups of lateral neurons (LN) and three groups of dorsal neurons govern behavioral rhythmicity of Drosophila. Recent data suggest that two groups of the LN (the ventral subset of the small LN cells and the dorsal subset of LN cells) are plausible candidates for the M and E oscillator, respectively. We provide evidence that these neuronal groups respond differently to light and can be completely desynchronized from one another by constant light, leading to two activity components that free-run with different periods. As expected, a long-period component started from the E activity bout. However, a short-period component originated not exclusively from the morning peak but more prominently from the evening peak. This reveals an interesting deviation from the original Pittendrigh and Daan (1976) model and suggests that a subgroup of the ventral subset of the small LN acts as "main" oscillator controlling M and E activity bouts in Drosophila.
Key words: period; timeless; dual oscillators; internal desynchronization; constant light; cryptochrome
Received March 30, 2005; revised Dec. 22, 2005; accepted Dec. 23, 2005.
Correspondence should be addressed to Charlotte Helfrich-Förster, University of Regensburg, Institute of Zoology, Universitaetsstraße 31, 93040 Regensburg, Germany. Email: charlotte.foerster{at}biologie.uni-regensburg./.de
This article has been cited by other articles:
T. Yoshii, S. Vanin, R. Costa, and C. Helfrich-Forster
Synergic Entrainment of Drosophila's Circadian Clock by Light and Temperature
J Biol Rhythms, December 1, 2009; 24(6): 452 - 464.
[Abstract] [PDF]
C. Helfrich-Forster
Does the Morning and Evening Oscillator Model Fit Better for Flies or Mice?
J Biol Rhythms, August 1, 2009; 24(4): 259 - 270.
[Abstract] [PDF]
D. Rieger, C. Wulbeck, F. Rouyer, and C. Helfrich-Forster
Period Gene Expression in Four Neurons Is Sufficient for Rhythmic Activity of Drosophila melanogaster under Dim Light Conditions
J Biol Rhythms, August 1, 2009; 24(4): 271 - 282.
[Abstract] [PDF]
H.-C. Hung, S. A. Kay, and F. Weber
HSP90, a Capacitor of Behavioral Variation
J Biol Rhythms, June 1, 2009; 24(3): 183 - 192.
[Abstract] [PDF]
S. Shiga and H. Numata
Roles of PER immunoreactive neurons in circadian rhythms and photoperiodism in the blow fly, Protophormia terraenovae
J. Exp. Biol., March 15, 2009; 212(6): 867 - 877.
[Abstract] [Full Text] [PDF]
J. H. Bahn, G. Lee, and J. H. Park
Comparative Analysis of Pdf-Mediated Circadian Behaviors Between Drosophila melanogaster and D. virilis
Genetics, March 1, 2009; 181(3): 965 - 975.
[Abstract] [Full Text] [PDF]
T. Yoshii, C. Wulbeck, H. Sehadova, S. Veleri, D. Bichler, R. Stanewsky, and C. Helfrich-Forster
The Neuropeptide Pigment-Dispersing Factor Adjusts Period and Phase of Drosophila's Clock
J. Neurosci., February 25, 2009; 29(8): 2597 - 2610.
[Abstract] [Full Text] [PDF]
C.-M. Lee, M.-T. Su, and H.-J. Lee
Pigment Dispersing Factor: An Output Regulator of the Circadian Clock in the German Cockroach
J Biol Rhythms, February 1, 2009; 24(1): 35 - 43.
[Abstract] [PDF]
Junwei Wang, Jiajun Zhang, Zhanjiang Yuan, Aimin Chen, and Tianshou Zhou
Neurotransmitter-Mediated Collective Rhythms in Grouped Drosophila Circadian Clocks
J Biol Rhythms, December 1, 2008; 23(6): 472 - 482.
[Abstract] [PDF]
C. Wulbeck, E. Grieshaber, and C. Helfrich-Forster
Pigment-Dispersing Factor (PDF) Has Different Effects on Drosophila's Circadian Clocks in the Accessory Medulla and in the Dorsal Brain
J Biol Rhythms, October 1, 2008; 23(5): 409 - 424.
[Abstract] [PDF]
J. Benito, J. H. Houl, G. W. Roman, and P. E. Hardin
The Blue-Light Photoreceptor CRYPTOCHROME Is Expressed in a Subset of Circadian Oscillator Neurons in the Drosophila CNS
J Biol Rhythms, August 1, 2008; 23(4): 296 - 307.
[Abstract] [PDF]
V. Sheeba, V. K. Sharma, H. Gu, Y.-T. Chou, D. K. O'Dowd, and T. C. Holmes
Pigment Dispersing Factor-Dependent and -Independent Circadian Locomotor Behavioral Rhythms
J. Neurosci., January 2, 2008; 28(1): 217 - 227.
[Abstract] [Full Text] [PDF]
J.-Y. Fan, M. J. Muskus, and J. L. Price
Entrainment of the Drosophila Circadian Clock: More Heat Than Light
Sci. Signal., November 20, 2007; 2007(413): pe65 - pe65.
[Abstract] [Full Text] [PDF]
V. Codd, D. Dolezel, J. Stehlik, A. Piccin, K. J. Garner, S. N. Racey, K. R. Straatman, E. J. Louis, R. Costa, I. Sauman, et al.
Circadian Rhythm Gene Regulation in the Housefly Musca domestica
Genetics, November 1, 2007; 177(3): 1539 - 1551.
[Abstract] [Full Text] [PDF]
A. Busza, A. Murad, and P. Emery
Interactions between Circadian Neurons Control Temperature Synchronization of Drosophila Behavior
J. Neurosci., October 3, 2007; 27(40): 10722 - 10733.
[Abstract] [Full Text] [PDF]
E. Dolezelova, D. Dolezel, and J. C. Hall
Rhythm Defects Caused by Newly Engineered Null Mutations in Drosophila's cryptochrome Gene
Genetics, September 1, 2007; 177(1): 329 - 345.
[Abstract] [Full Text] [PDF]
C. Lim, J. Lee, C. Choi, J. Kim, E. Doh, and J. Choe
Functional Role of CREB-Binding Protein in the Circadian Clock System of Drosophila melanogaster
Mol. Cell. Biol., July 1, 2007; 27(13): 4876 - 4890.
[Abstract] [Full Text] [PDF]
Y. Miyasako, Y. Umezaki, and K. Tomioka
Separate Sets of Cerebral Clock Neurons Are Responsible for Light and Temperature Entrainment of Drosophila Circadian Locomotor Rhythms
J Biol Rhythms, April 1, 2007; 22(2): 115 - 126.
[Abstract] [PDF]
W. Bachleitner, L. Kempinger, C. Wulbeck, D. Rieger, and C. Helfrich-Forster
Moonlight shifts the endogenous clock of Drosophila melanogaster
PNAS, February 27, 2007; 104(9): 3538 - 3543.
[Abstract] [Full Text] [PDF]
C. Helfrich-Forster, T. Yoshii, C. Wulbeck, E. Grieshaber, D. Rieger, W. Bachleitner, P. Cusumano, and F. Rouyer
The Lateral and Dorsal Neurons of Drosophila melanogaster: New Insights about Their Morphology and Function
Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 517 - 525.
[Abstract] [PDF]
P. H. Taghert and O. T. Shafer
Mechanisms of Clock Output in the Drosophila Circadian Pacemaker System
J Biol Rhythms, December 1, 2006; 21(6): 445 - 457.
[Abstract] [PDF]
|
|
|
|
 |
|