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The Journal of Neuroscience, April 11, 2007, 27(15):4110-4119; doi:10.1523/JNEUROSCI.0025-07.2007
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Cellular/Molecular
An Intersubunit Trigger of Channel Gating in the Muscle Nicotinic Receptor
Nuriya Mukhtasimova andSteven M. Sine Departments of Physiology and Biomedical Engineering and Neurology, Receptor Biology Laboratory, Mayo Clinic College of Medicine, Rochester, Minnesota 55905
Correspondence should be addressed to Steven M. Sine, Departments of Physiology and Biomedical Engineering and Neurology, Receptor Biology Laboratory, Mayo Clinic College of Medicine, Rochester, MN 55905. Email: sine.steven{at}mayo.edu
Binding of neurotransmitter triggers gating of synaptic receptor channels, but our understanding of the structures that link the binding site to the channel is just beginning to develop. Here, we identify an intersubunit triggering element required for rapid and efficient gating of muscle nicotinic receptors using a structural model of the Torpedo receptor at 4 Å resolution, recordings of currents through single receptor channels, measurements of inter-residue energetic coupling, and functional consequences of disulfide trapping. Mutation of the conserved residues,
Tyr 127,
Asn 39, and
Asn 41, located at the two subunit interfaces that form the agonist binding sites, markedly attenuates acetylcholine-elicited channel gating; mutant cycle analyses based on changes in the channel gating equilibrium constant reveal strong energetic coupling among these residues. After each residue is substituted with Cys, oxidizing conditions that promote disulfide bond formation attenuate gating of mutant, but not wild-type receptors. Gating is similarly attenuated when the Cys substitutions are confined to either of the binding-site interfaces, but can be restored by reducing conditions that promote disulfide bond breakage. Thus, the Tyr–Asn pair is an intersubunit trigger of rapid and efficient gating of muscle nicotinic receptors.
Key words: acetylcholine receptor; channel gating; single channel kinetics; intersubunit trigger; coupling energy; binding
Received Jan. 4, 2007; revised March 1, 2007; accepted March 2, 2007.
Correspondence should be addressed to Steven M. Sine, Departments of Physiology and Biomedical Engineering and Neurology, Receptor Biology Laboratory, Mayo Clinic College of Medicine, Rochester, MN 55905. Email: sine.steven{at}mayo.edu
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