QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, May 9, 2007, 27(19):5146-5155; doi:10.1523/JNEUROSCI.0654-07.2007

This Article Full Text Full Text (PDF) Supplemental Data Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (21) Citing Articles via Google Scholar Google Scholar Articles by Young, K. M. Articles by Bartlett, P. F. Search for Related Content PubMed PubMed Citation Articles by Young, K. M. Articles by Bartlett, P. F.

 Previous Article  |  Next Article 

Development/Plasticity/Repair
p75 Neurotrophin Receptor Expression Defines a Population of BDNF-Responsive Neurogenic Precursor Cells

Kaylene M. Young,^1,2 Tobias D. Merson,³ Areechun Sotthibundhu,¹ Elizabeth J. Coulson,^1 * and Perry F. Bartlett^1 *

¹Queensland Brain Institute, The University of Queensland, Brisbane QLD 4072, Australia, ²The Walter and Eliza Hall Institute for Medical Research, Melbourne VIC 3050, Australia, and ³Howard Florey Institute, The University of Melbourne, Melbourne VIC 3010, Australia

Correspondence should be addressed to Perry F. Bartlett, Queensland Brain Institute, The University of Queensland, Brisbane QLD 4072, Australia. Email: p.bartlett{at}uq.edu.au

Although our understanding of adult neurogenesis has increased dramatically over the last decade, confusion still exists regarding both the identity of the stem cell responsible for neuron production and the mechanisms that regulate its activity. Here we show, using flow cytometry, that a small population of cells (0.3%) within the stem cell niche of the rat subventricular zone (SVZ) expresses the p75 neurotrophin receptor (p75^NTR) and that these cells are responsible for neuron production in both newborn and adult animals. In the adult, the p75^NTR-positive population contains all of the neurosphere-producing precursor cells, whereas in the newborn many of the precursor cells are p75^NTR negative. However, at both ages, only the neurospheres derived from p75^NTR-positive cells are neurogenic. We also show that neuron production from p75^NTR-positive but not p75^NTR-negative precursors is greatly enhanced after treatment with brain-derived neurotrophic factor (BDNF) or nerve growth factor. This effect appears to be mediated specifically by p75^NTR, because precursor cells from p75^NTR-deficient mice show a 70% reduction in their neurogenic potential in vitro and fail to respond to BDNF treatment. Furthermore, adult p75^NTR-deficient mice have significantly reduced numbers of PSA-NCAM (polysialylated neural cell adhesion molecule)-positive SVZ neuroblasts in vivo and a lower olfactory bulb weight. Thus, p75^NTR defines a discrete population of highly proliferative SVZ precursor cells that are able to respond to neurotrophin activation by increasing neuroblast generation, making this pathway the most likely mechanism for the increased neurogenesis that accompanies raised BDNF levels in a variety of disease and behavioral situations.

Key words: subventricular zone (SVZ); BDNF; stem cell; neurotrophin; p75 neurotrophin receptor (p75^NTR); neurogenesis

---

Received Oct. 6, 2005; revised March 30, 2007; accepted March 30, 2007.

Correspondence should be addressed to Perry F. Bartlett, Queensland Brain Institute, The University of Queensland, Brisbane QLD 4072, Australia. Email: p.bartlett{at}uq.edu.au

This article has been cited by other articles:

				I. Kazanis The subependymal zone neurogenic niche: a beating heart in the centre of the brain: How plastic is adult neurogenesis? Opportunities for therapy and questions to be addressed Brain, November 1, 2009; 132(11): 2909 - 2921. [Abstract] [Full Text] [PDF]

				B. Paradiso, P. Marconi, S. Zucchini, E. Berto, A. Binaschi, A. Bozac, A. Buzzi, M. Mazzuferi, E. Magri, G. N. Mora, et al. Localized delivery of fibroblast growth factor-2 and brain-derived neurotrophic factor reduces spontaneous seizures in an epilepsy model PNAS, April 28, 2009; 106(17): 7191 - 7196. [Abstract] [Full Text] [PDF]

				M. Snapyan, M. Lemasson, M. S. Brill, M. Blais, M. Massouh, J. Ninkovic, C. Gravel, F. Berthod, M. Gotz, P. A. Barker, et al. Vasculature Guides Migrating Neuronal Precursors in the Adult Mammalian Forebrain via Brain-Derived Neurotrophic Factor Signaling J. Neurosci., April 1, 2009; 29(13): 4172 - 4188. [Abstract] [Full Text] [PDF]

				T. Hagg From Neurotransmitters to Neurotrophic Factors to Neurogenesis Neuroscientist, February 1, 2009; 15(1): 20 - 27. [Abstract] [PDF]

				R. P. Galvao, J. M. Garcia-Verdugo, and A. Alvarez-Buylla Brain-Derived Neurotrophic Factor Signaling Does Not Stimulate Subventricular Zone Neurogenesis in Adult Mice and Rats J. Neurosci., December 10, 2008; 28(50): 13368 - 13383. [Abstract] [Full Text] [PDF]

				T.-F. Yuan BDNF Signaling during Olfactory Bulb Neurogenesis J. Neurosci., May 14, 2008; 28(20): 5139 - 5140. [Full Text] [PDF]

				T. L. Walker, A. White, D. M. Black, R. H. Wallace, P. Sah, and P. F. Bartlett Latent Stem and Progenitor Cells in the Hippocampus Are Activated by Neural Excitation J. Neurosci., May 14, 2008; 28(20): 5240 - 5247. [Abstract] [Full Text] [PDF]

				K. G. Bath, N. Mandairon, D. Jing, R. Rajagopal, R. Kapoor, Z.-Y. Chen, T. Khan, C. C. Proenca, R. Kraemer, T. A. Cleland, et al. Variant Brain-Derived Neurotrophic Factor (Val66Met) Alters Adult Olfactory Bulb Neurogenesis and Spontaneous Olfactory Discrimination J. Neurosci., March 5, 2008; 28(10): 2383 - 2393. [Abstract] [Full Text] [PDF]

				D. Colak, T. Mori, M. S. Brill, A. Pfeifer, S. Falk, C. Deng, R. Monteiro, C. Mummery, L. Sommer, and M. Gotz Adult Neurogenesis Requires Smad4-Mediated Bone Morphogenic Protein Signaling in Stem Cells J. Neurosci., January 9, 2008; 28(2): 434 - 446. [Abstract] [Full Text] [PDF]

				K. M. Young, M. Fogarty, N. Kessaris, and W. D. Richardson Subventricular Zone Stem Cells Are Heterogeneous with Respect to Their Embryonic Origins and Neurogenic Fates in the Adult Olfactory Bulb J. Neurosci., August 1, 2007; 27(31): 8286 - 8296. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help