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The Journal of Neuroscience, May 9, 2007, 27(19):5215-5223; doi:10.1523/JNEUROSCI.4685-06.2007

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Development/Plasticity/Repair
Interplay between Laminar Specificity and Activity-Dependent Mechanisms of Thalamocortical Axon Branching

Naofumi Uesaka, ^* Yasufumi Hayano, ^* Akito Yamada, and Nobuhiko Yamamoto

Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan

Correspondence should be addressed to Nobuhiko Yamamoto, Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan. Email: nobuhiko{at}fbs.osaka-u.ac.jp

Target and activity-dependent mechanisms of axonal branching were studied in the thalamocortical (TC) projection using organotypic cocultures of the thalamus and cortex. TC axons were labeled with enhanced yellow fluorescent protein (EYFP) by a single-cell electroporation method and observed over time by confocal microscopy. Changes in the firing activity of cocultures grown on multielectrode dishes were also monitored over time. EYFP-labeled TC axons exhibited more branch formation in and around layer 4 of the cortical explant during the second week in vitro, when spontaneous firing activity increased in both thalamic and cortical cells. Time-lapse imaging further demonstrated that branching patterns were generated dynamically by addition and elimination with a bias toward branch accumulation in the target layer. To examine the relationship between neural activity and TC branch formation, the dynamics of axonal branching was analyzed under various pharmacological treatments. Chronic blockade of firing or synaptic activity reduced the remodeling process, in particular, branch addition in the target layer. However, extension of branches was not affected by this treatment. Together, these findings suggest that neural activity can modify the molecular mechanisms that regulate lamina-specific TC axon branching.

Key words: activity; axon guidance; cerebral cortex; lamina; thalamus; tissue culture

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Received Oct. 27, 2006; revised April 9, 2007; accepted April 11, 2007.

Correspondence should be addressed to Nobuhiko Yamamoto, Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan. Email: nobuhiko{at}fbs.osaka-u.ac.jp

This article has been cited by other articles:

				Y. Hayano and N. Yamamoto Activity-Dependent Thalamocortical Axon Branching Neuroscientist, August 1, 2008; 14(4): 359 - 368. [Abstract] [PDF]

				B. I. Hutchins and K. Kalil Differential Outgrowth of Axons and their Branches Is Regulated by Localized Calcium Transients J. Neurosci., January 2, 2008; 28(1): 143 - 153. [Abstract] [Full Text] [PDF]

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