QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, August 15, 2007, 27(33):8845-8856; doi:10.1523/JNEUROSCI.0551-07.2007

This Article Full Text Full Text (PDF) Supplemental Data Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by Berg, A. P. Articles by Bayliss, D. A. Search for Related Content PubMed PubMed Citation Articles by Berg, A. P. Articles by Bayliss, D. A.

 Previous Article  |  Next Article 

Cellular/Molecular
TrpC3/C7 and Slo2.1 Are Molecular Targets for Metabotropic Glutamate Receptor Signaling in Rat Striatal Cholinergic Interneurons

Allison P. Berg,¹ Neil Sen,¹ and Douglas A. Bayliss^1,2

Departments of ¹Pharmacology and ²Anesthesiology, University of Virginia, Charlottesville, Virginia 22908

Correspondence should be addressed to Allison P. Berg at her present address: Feinberg School of Medicine, Northwestern University, 303 East Chicago Avenue, Chicago, IL 60611-3008. Email: a-berg{at}northwestern.edu

Large aspiny cholinergic interneurons provide the sole source of striatal acetylcholine, a neurotransmitter critical for basal ganglia function; these tonically active interneurons receive excitatory inputs from corticostriatal glutamatergic afferents that act, in part, via metabotropic glutamate receptors (mGluRs). We combined electrophysiological recordings in brain slices with molecular neuroanatomy to identify distinct ion channel targets for mGluR1/5 receptors in striatal cholinergic interneurons: transient receptor potential channel 3/7 (TrpC3/C7) and Slo2.1. In recordings obtained with methanesulfonate-based internal solutions, we found an mGluR-activated current with voltage-dependent and pharmacological properties reminiscent of TrpC3 and TrpC7; expression of these TrpC subunits in cholinergic interneurons was verified by combined immunohistochemistry and in situ hybridization, and modulation of both TrpC channels was reconstituted in HEK293 (human embryonic kidney 293) cells cotransfected with mGluR1 or mGluR5. With a chloride-based internal solution, mGluR agonists did not activate interneuron TrpC-like currents. Instead, a time-dependent, outwardly rectifying K⁺ current developed after whole-cell access, and this Cl^–-activated K⁺ current was strongly inhibited by volatile anesthetics and mGluR activation. This modulation was recapitulated in cells transfected with Slo2.1, a Na⁺- and Cl^–-activated K⁺ channel, and Slo2.1 expression was confirmed histochemically in striatal cholinergic interneurons. By using gramicidin perforated-patch recordings, we established that the predominant agonist-activated current was TrpC-like when ambient intracellular chloride was preserved, although a small K⁺ current contribution was observed in some cells. Together, our data indicate that mGluR1/5-mediated glutamatergic excitation of cholinergic interneurons is primarily a result of activation of TrpC3/TrpC7-like cationic channels; under conditions when intracellular NaCl is elevated, a Slo2.1 background K⁺ channel may also contribute.

Key words: mGluR; TrpC; Slo2; striatum; interneurons; basal ganglia

---

Received Feb. 7, 2007; revised July 2, 2007; accepted July 2, 2007.

Correspondence should be addressed to Allison P. Berg at her present address: Feinberg School of Medicine, Northwestern University, 303 East Chicago Avenue, Chicago, IL 60611-3008. Email: a-berg{at}northwestern.edu

This article has been cited by other articles:

				X. Chen, S. Shu, L. C. Schwartz, C. Sun, J. Kapur, and D. A. Bayliss Homeostatic Regulation of Synaptic Excitability: Tonic GABAA Receptor Currents Replace Ih in Cortical Pyramidal Neurons of HCN1 Knock-Out Mice J. Neurosci., February 17, 2010; 30(7): 2611 - 2622. [Abstract] [Full Text] [PDF]

				E. Nanou and A. El Manira Mechanisms of Modulation of AMPA-Induced Na+-Activated K+ Current by mGluR1 J Neurophysiol, January 1, 2010; 103(1): 441 - 445. [Abstract] [Full Text] [PDF]

				P. Deng, Z.-P. Pang, Z. Lei, and Z. C. Xu Excitatory Roles of Protein Kinase C in Striatal Cholinergic Interneurons J Neurophysiol, October 1, 2009; 102(4): 2453 - 2461. [Abstract] [Full Text] [PDF]

				H.-W. Dong, A. Hayar, J. Callaway, X.-H. Yang, Q. Nai, and M. Ennis Group I mGluR Activation Enhances Ca2+-Dependent Nonselective Cation Currents and Rhythmic Bursting in Main Olfactory Bulb External Tufted Cells J. Neurosci., September 23, 2009; 29(38): 11943 - 11953. [Abstract] [Full Text] [PDF]

				T. Collin, R. Franconville, B. E. Ehrlich, and I. Llano Activation of Metabotropic Glutamate Receptors Induces Periodic Burst Firing and Concomitant Cytosolic Ca2+ Oscillations in Cerebellar Interneurons J. Neurosci., July 22, 2009; 29(29): 9281 - 9291. [Abstract] [Full Text] [PDF]

				J. Abramowitz and L. Birnbaumer Physiology and pathophysiology of canonical transient receptor potential channels FASEB J, February 1, 2009; 23(2): 297 - 328. [Abstract] [Full Text] [PDF]

				C. Zhang, T. A. Roepke, M. J. Kelly, and O. K. Ronnekleiv Kisspeptin Depolarizes Gonadotropin-Releasing Hormone Neurons through Activation of TRPC-Like Cationic Channels J. Neurosci., April 23, 2008; 28(17): 4423 - 4434. [Abstract] [Full Text] [PDF]

				D. K. Mulkey, E. M. Talley, R. L. Stornetta, A. R. Siegel, G. H. West, X. Chen, N. Sen, A. M. Mistry, P. G. Guyenet, and D. A. Bayliss TASK Channels Determine pH Sensitivity in Select Respiratory Neurons But Do Not Contribute to Central Respiratory Chemosensitivity J. Neurosci., December 19, 2007; 27(51): 14049 - 14058. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help