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The Journal of Neuroscience, September 5, 2007, 27(36):9670-9681; doi:10.1523/JNEUROSCI.2189-07.2007

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Development/Plasticity/Repair
Fate-Mapping the Mammalian Hindbrain: Segmental Origins of Vestibular Projection Neurons Assessed Using Rhombomere-Specific Hoxa2 Enhancer Elements in the Mouse Embryo

Massimo Pasqualetti,^1 * Carmen Díaz,^2 * Jean-Sébastien Renaud,^3 * Filippo M. Rijli,¹  and Joel C. Glover³ 

¹Institut de Génétique et de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique/Inserm/Université Louis Pasteur, Unité Mixte de Recherche 7104, Commanaute Urbaine de Strasbourg, 67404 Illkirch Cedex, France, ²Faculty of Medicine, University of Castilla-La Mancha, Regional Center for Biomedical Science, 02071 Albacete, Spain, and ³Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway

Correspondence should be addressed to Dr. Joel C. Glover, Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, Post Box 1103 Blindern, 0317 Oslo, Norway. Email: joel.glover{at}medisin.uio.no

As a step toward generating a fate map of identified neuron populations in the mammalian hindbrain, we assessed the contributions of individual rhombomeres to the vestibular nuclear complex, a major sensorimotor area that spans the entire rhombencephalon. Transgenic mice harboring either the lacZ or the enhanced green fluorescent protein reporter genes under the transcriptional control of rhombomere-specific Hoxa2 enhancer elements were used to visualize rhombomere-derived domains. We labeled functionally identifiable vestibular projection neuron groups retrogradely with conjugated dextran-amines at successive embryonic stages and obtained developmental fate maps through direct comparison with the rhombomere-derived domains in the same embryos. The fate maps show that each vestibular neuron group derives from a unique rostrocaudal domain that is relatively stable developmentally, suggesting that anteroposterior migration is not a major contributor to the rostrocaudal patterning of the vestibular system. Most of the groups are multisegmental in origin, and each rhombomere is fated to give rise to two or more vestibular projection neuron types, in a complex pattern that is not segmentally iterated. Comparison with studies in the chicken embryo shows that the rostrocaudal patterning of identified vestibular projection neuron groups is generally well conserved between avians and mammalians but that significant species-specific differences exist in the rostrocaudal limits of particular groups. This mammalian hindbrain fate map can be used as the basis for targeting genetic manipulation to specific subpopulations of vestibular projection neurons.

Key words: lineage; brainstem; patterning; Cre-transgenic; neuromere; rhombencephalon; patterning

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Received May 13, 2007; revised July 15, 2007; accepted July 18, 2007.

Correspondence should be addressed to Dr. Joel C. Glover, Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, Post Box 1103 Blindern, 0317 Oslo, Norway. Email: joel.glover{at}medisin.uio.no

This article has been cited by other articles:

				K. Szokol, J. C. Glover, and M.-C. Perreault Differential origin of reticulospinal drive to motoneurons innervating trunk and hindlimb muscles in the mouse revealed by optical recording J. Physiol., November 1, 2008; 586(21): 5259 - 5276. [Abstract] [Full Text] [PDF]

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