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The Journal of Neuroscience, September 26, 2007, 27(39):10323-10332; doi:10.1523/JNEUROSCI.1102-07.2007

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Cellular/Molecular
Trio Controls the Mature Organization of Neuronal Clusters in the Hindbrain

Stéphanie Backer,^1,2 Matías Hidalgo-Sánchez,^1,2 Nicolas Offner,^1,2 Elodie Portales-Casamar,³ Anne Debant,³ Philippe Fort,³ Cécile Gauthier-Rouvière,³ and Evelyne Bloch-Gallego^1,2

¹Institut Cochin, Université Paris Descartes, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche 8104, 75014 Paris, France, ²Inserm, Unité 567, 75014 Paris, France, and ³Centre de Recherche de Biochimie Macromoléculaire/CNRS, Formation de Recherche en Evolution 2593, 34293 Montpellier Cedex, France

Correspondence should be addressed to Evelyne Bloch-Gallego, Institut Cochin, Département Génétique et Développement, 24 Rue du Faubourg Saint Jacques, 75014 Paris, France. Email: bloch-gallego{at}cochin.inserm.fr

During the embryonic development of the hindbrain, movements of neuronal clusters allow the formation of mature "pools", in particular for inferior olivary (ION) and facial motor (fMN) nuclei. The cellular mechanisms of neuron clustering remain uncharacterized. We report that the absence of the Rho–guanine exchange factor Trio, which can activate both RhoG and Rac1 in vivo, prevents the proper formation of ION and fMN subnuclei. Rac1, but not RhoG, appears to be a downstream actor in Trio-induced lamellation. In addition, we report that Cadherin-11 is expressed by a subset of neurons through the overall period of ION and fMN parcellations, and defects observed in trio mutant mice are located specifically in Cadherin-11-expressing regions. Moreover, endogenous Cadherin-11 is found in a complex with Trio when lamellation occurs. Altogether, those results establish a link between Trio activity, the subsequent Rac1 activation, and neuronal clusters organization, as well as a possible recruitment of the Cadherin-11 adhesive receptor to form a complex with Trio.

Key words: Rho GTPases; Cadherin-11; in situ hybridization; mice; motoneurons; olivary neurons

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Received March 12, 2007; revised July 3, 2007; accepted July 3, 2007.

Correspondence should be addressed to Evelyne Bloch-Gallego, Institut Cochin, Département Génétique et Développement, 24 Rue du Faubourg Saint Jacques, 75014 Paris, France. Email: bloch-gallego{at}cochin.inserm.fr

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