QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, November 28, 2007, 27(48):13098-13107; doi:10.1523/JNEUROSCI.3986-07.2007

This Article Full Text Full Text (PDF) Supplemental Data Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (8) Citing Articles via Google Scholar Google Scholar Articles by Terabayashi, T. Articles by Miki, H. Search for Related Content PubMed PubMed Citation Articles by Terabayashi, T. Articles by Miki, H.

 Previous Article  |  Next Article 

Cellular/Molecular
Polarity-Regulating Kinase Partitioning-Defective 1/Microtubule Affinity-Regulating Kinase 2 Negatively Regulates Development of Dendrites on Hippocampal Neurons

Takeshi Terabayashi,¹ Tomohiko J. Itoh,² Hideki Yamaguchi,³ Yuta Yoshimura,¹ Yosuke Funato,¹ Shigeo Ohno,⁴ and Hiroaki Miki¹

¹Laboratory of Intracellular Signaling, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan, ²Division of Biological Sciences, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan, ³Laboratory of Genome and Biosignal, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan, and ⁴Department of Molecular Biology, Yokohama City University School of Medicine, Kanazawa-ku, Yokohama 236-0004, Japan

Correspondence should be addressed to Hiroaki Miki, Laboratory of Intracellular Signaling, Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan. Email: hmiki{at}protein.osaka-u.ac.jp

Neurons are highly polarized cells that possess two morphologically and functionally different types of protrusions, axons and dendrites, that function in the transmission and reception of neural signals, respectively. A great deal of attention has been paid to the specification and guidance of axons, but the mechanism of dendrite development remains mostly unknown. We report here that a polarity-regulating kinase, partitioning-defective 1 (Par1b)/microtubule affinity-regulating kinase 2 (MARK2), specifically regulates development of dendrites in hippocampal neurons. Ectopic expression of Par1b/MARK2 shortens the length and decreases branching of dendrites without significant effects on axons. Knockdown of endogenous Par1b/MARK2 by RNA interference stimulates dendrite development. Wnt stimulation and Dishevelled expression, both of which are known to induce dendrite development, induced recruitment of Par1b/MARK2 to the membrane fraction. Expression of a Par1b/MARK2 mutant, that contains a myristoylation signal and accumulates exclusively in membranes, does not affect dendrite development. In addition, Par1b/MARK2 efficiently phosphorylated MAP2, which is localized mainly in dendrites. These results indicate that Par1b/MARK2 negatively regulates dendrite development through phosphorylation of MAP2.

Key words: dendrite; Wnt; Par1b/MARK2; dishevelled; translocation; MAP2

---

Received Dec. 3, 2006; revised Oct. 11, 2007; accepted Oct. 11, 2007.

Correspondence should be addressed to Hiroaki Miki, Laboratory of Intracellular Signaling, Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan. Email: hmiki{at}protein.osaka-u.ac.jp

This article has been cited by other articles:

				T. Terabayashi, Y. Funato, M. Fukuda, and H. Miki A Coated Vesicle-associated Kinase of 104 kDa (CVAK104) Induces Lysosomal Degradation of Frizzled 5 (Fzd5) J. Biol. Chem., September 25, 2009; 284(39): 26716 - 26724. [Abstract] [Full Text] [PDF]

				J. Lin, K. K. Hou, H. Piwnica-Worms, and A. S. Shaw The Polarity Protein Par1b/EMK/MARK2 Regulates T Cell Receptor-Induced Microtubule-Organizing Center Polarization J. Immunol., July 15, 2009; 183(2): 1215 - 1221. [Abstract] [Full Text] [PDF]

				T. Sapir, A. Shmueli, T. Levy, T. Timm, M. Elbaum, E.-M. Mandelkow, and O. Reiner Antagonistic Effects of Doublecortin and MARK2/Par-1 in the Developing Cerebral Cortex J. Neurosci., November 26, 2008; 28(48): 13008 - 13013. [Abstract] [Full Text] [PDF]

				Y. Funato, T. Michiue, T. Terabayashi, A. Yukita, H. Danno, M. Asashima, and H. Miki Nucleoredoxin regulates the Wnt/planar cell polarity pathway in Xenopus Genes Cells, September 1, 2008; 13(9): 965 - 975. [Abstract] [Full Text] [PDF]

				G. Maussion, J. Carayol, A.-M. Lepagnol-Bestel, F. Tores, Y. Loe-Mie, U. Milbreta, F. Rousseau, K. Fontaine, J. Renaud, J.-M. Moalic, et al. Convergent evidence identifying MAP/microtubule affinity-regulating kinase 1 (MARK1) as a susceptibility gene for autism Hum. Mol. Genet., August 15, 2008; 17(16): 2541 - 2551. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help