QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, November 28, 2007, 27(48):13329-13340; doi:10.1523/JNEUROSCI.4083-07.2007

This Article Full Text Full Text (PDF) Supplemental Data Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Kang, H. J. Articles by Duman, R. S. Search for Related Content PubMed PubMed Citation Articles by Kang, H. J. Articles by Duman, R. S.

 Previous Article  |  Next Article 

Neurobiology of Disease
Gene Expression Profiling in Postmortem Prefrontal Cortex of Major Depressive Disorder

Hyo Jung Kang,¹ David H. Adams,¹ Arthur Simen,¹ Birgitte B. Simen,¹ Grazyna Rajkowska,² Craig A. Stockmeier,^2,3 James C. Overholser,⁴ Herbert Y. Meltzer,⁵ George J. Jurjus,³ Lisa C. Konick,³ Samuel S. Newton,¹ and Ronald S. Duman¹

¹Department Psychiatry, Yale University, New Haven, Connecticut 06508, ²Department Psychiatry and Human Behavior, University of Mississippi Medical Center, Jackson, Mississippi 39216, Departments of ³Psychiatry and ⁴Psychology, Case Western Reserve University, Cleveland, Ohio 44106, and ⁵Psychiatric Hospital at Vanderbilt, Nashville, Tennessee 37212

Correspondence should be addressed to Ronald S. Duman, Department of Psychiatry, Yale University School of Medicine, New Haven, CT 06511. Email: ronald.duman{at}yale.edu

Investigations of the molecular mechanisms underlying major depressive disorder (MDD) have been hampered by the complexity of brain tissue and sensitivity of gene expression profiling approaches. To address these issues, we used discrete microdissections of postmortem dorsolateral prefrontal cortex (DLPFC) (area 9) and an oligonucleotide (60mer) microarray hybridization procedure that increases sensitivity without RNA amplification. Mixed-effects statistical methods were used to rigorously control for medication usage in the subset of medicated depressed subjects. These analyses yielded a rich profile of dysregulated genes. Two of the most highly dysregulated genes of interest were stresscopin, a neuropeptide involved in stress responses, and Forkhead box D3 (FOXD3), a transcription factor. Secondary cell-based analysis demonstrated that stresscopin and FoxD3 are increased in neurons of DLPFC gray matter of MDD subjects. These findings identify abnormal gene expression in a discrete region of MDD subjects and contribute to further elucidation of the molecular alterations of this complex mood disorder.

Key words: microarray; stresscopin; urocortin III; stress; corticotrophin releasing hormone; FoxD3; fibroblast growth factor

---

Received June 11, 2007; revised Oct. 15, 2007; accepted Oct. 16, 2007.

Correspondence should be addressed to Ronald S. Duman, Department of Psychiatry, Yale University School of Medicine, New Haven, CT 06511. Email: ronald.duman{at}yale.edu

This article has been cited by other articles:

				E. Sibille, Y. Wang, J. Joeyen-Waldorf, C. Gaiteri, A. Surget, S. Oh, C. Belzung, G. C. Tseng, and D. A. Lewis A Molecular Signature of Depression in the Amygdala Am J Psychiatry, September 1, 2009; 166(9): 1011 - 1024. [Abstract] [Full Text] [PDF]

				J. Fassunke, M. Majores, A. Tresch, P. Niehusmann, A. Grote, S. Schoch, and A. J. Becker Array analysis of epilepsy-associated gangliogliomas reveals expression patterns related to aberrant development of neuronal precursors Brain, November 1, 2008; 131(11): 3034 - 3050. [Abstract] [Full Text] [PDF]

				J. G. McEuen, S. G. Beck, and T. L. Bale Failure to Mount Adaptive Responses to Stress Results in Dysregulation and Cell Death in the Midbrain Raphe J. Neurosci., August 13, 2008; 28(33): 8169 - 8177. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help