QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, December 19, 2007, 27(51):14049-14058; doi:10.1523/JNEUROSCI.4254-07.2007

This Article Full Text Full Text (PDF) Supplemental Data Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (23) Citing Articles via Google Scholar Google Scholar Articles by Mulkey, D. K. Articles by Bayliss, D. A. Search for Related Content PubMed PubMed Citation Articles by Mulkey, D. K. Articles by Bayliss, D. A.

 Previous Article  |  Next Article 

Behavioral/Systems/Cognitive
TASK Channels Determine pH Sensitivity in Select Respiratory Neurons But Do Not Contribute to Central Respiratory Chemosensitivity

Daniel K. Mulkey, ^* Edmund M. Talley, ^* Ruth L. Stornetta, Audra R. Siegel, Gavin H. West, Xiangdong Chen, Neil Sen, Akshitkumar M. Mistry, Patrice G. Guyenet, and Douglas A. Bayliss

Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908

Correspondence should be addressed to Douglas A. Bayliss, Department of Pharmacology, University of Virginia, Charlottesville, VA 22908-0735. Email: dab3y{at}virginia.edu

Central respiratory chemoreception is the mechanism by which the CNS maintains physiologically appropriate pH and PCO₂ via control of breathing. A prominent hypothesis holds that neural substrates for this process are distributed widely in the respiratory network, especially because many neurons that make up this network are chemosensitive in vitro. We and others have proposed that TASK channels (TASK-1, K_2P3.1 and/or TASK-3, K_2P9.1) may serve as molecular sensors for central chemoreception because they are highly expressed in multiple neuronal populations in the respiratory pathway and contribute to their pH sensitivity in vitro. To test this hypothesis, we examined the chemosensitivity of two prime candidate chemoreceptor neurons in vitro and tested ventilatory responses to CO₂ using TASK channel knock-out mice. The pH sensitivity of serotonergic raphe neurons was abolished in TASK channel knock-outs. In contrast, pH sensitivity of neurons in the mouse retrotrapezoid nucleus (RTN) was fully maintained in a TASK null background, and pharmacological evidence indicated that a K⁺ channel with properties distinct from TASK channels contributes to the pH sensitivity of rat RTN neurons. Furthermore, the ventilatory response to CO₂ was completely retained in single or double TASK knock-out mice. These data rule out a strict requirement for TASK channels or raphe neurons in central respiratory chemosensation. Furthermore, they indicate that a non-TASK K⁺ current contributes to chemosensitivity of RTN neurons, which are profoundly pH-sensitive and capable of driving respiratory output in response to local pH changes in vivo.

Key words: background potassium channel; KCNK; raphe; RTN; pH signaling; brain slice; plethysmography

---

Received Sept. 17, 2007; revised Nov. 8, 2007; accepted Nov. 8, 2007.

Correspondence should be addressed to Douglas A. Bayliss, Department of Pharmacology, University of Virginia, Charlottesville, VA 22908-0735. Email: dab3y{at}virginia.edu

This article has been cited by other articles:

				A. Kollewe, A. Y. Lau, A. Sullivan, Benoit Roux, and S. A.N. Goldstein A structural model for K2P potassium channels based on 23 pairs of interacting sites and continuum electrostatics J. Gen. Physiol., July 1, 2009; 134(1): 53 - 68. [Abstract] [Full Text] [PDF]

				S. Bittner, S. G. Meuth, K. Gobel, N. Melzer, A. M. Herrmann, O. J. Simon, A. Weishaupt, T. Budde, D. A. Bayliss, M. Bendszus, et al. TASK1 modulates inflammation and neurodegeneration in autoimmune inflammation of the central nervous system Brain, July 1, 2009; (2009) awp163v1. [Abstract] [Full Text] [PDF]

				C. N. Wyatt and C. Peers Hetero or homo, hypoxia has them all J. Physiol., June 15, 2009; 587(12): 2717 - 2718. [Full Text] [PDF]

				D. Kim, E. J. Cavanaugh, I. Kim, and J. L. Carroll Heteromeric TASK-1/TASK-3 is the major oxygen-sensitive background K+ channel in rat carotid body glomus cells J. Physiol., June 15, 2009; 587(12): 2963 - 2975. [Abstract] [Full Text] [PDF]

				S. B. G. Abbott, R. L. Stornetta, M. G. Fortuna, S. D. Depuy, G. H. West, T. E. Harris, and P. G. Guyenet Photostimulation of Retrotrapezoid Nucleus Phox2b-Expressing Neurons In Vivo Produces Long-Lasting Activation of Breathing in Rats J. Neurosci., May 6, 2009; 29(18): 5806 - 5819. [Abstract] [Full Text] [PDF]

				M. Zuzarte, K. Heusser, V. Renigunta, G. Schlichthorl, S. Rinne, E. Wischmeyer, J. Daut, B. Schwappach, and R. Preisig-Muller Intracellular traffic of the K+ channels TASK-1 and TASK-3: role of N- and C-terminal sorting signals and interaction with 14-3-3 proteins J. Physiol., March 1, 2009; 587(5): 929 - 952. [Abstract] [Full Text] [PDF]

				A. Guyon, M. P. Tardy, C. Rovere, J.-L. Nahon, J. Barhanin, and F. Lesage Glucose Inhibition Persists in Hypothalamic Neurons Lacking Tandem-Pore K+ Channels J. Neurosci., February 25, 2009; 29(8): 2528 - 2533. [Abstract] [Full Text] [PDF]

				K. M. Spyer To breathe or not to breathe? That is the question Exp Physiol, January 1, 2009; 94(1): 1 - 10. [Abstract] [Full Text] [PDF]

				H. Onimaru, K. Ikeda, and K. Kawakami CO2-Sensitive Preinspiratory Neurons of the Parafacial Respiratory Group Express Phox2b in the Neonatal Rat J. Neurosci., November 26, 2008; 28(48): 12845 - 12850. [Abstract] [Full Text] [PDF]

				A.-M. Linden, M. I. Aller, E. Leppa, P. H. Rosenberg, W. Wisden, and E. R. Korpi K+ Channel TASK-1 Knockout Mice Show Enhanced Sensitivities to Ataxic and Hypnotic Effects of GABAA Receptor Ligands J. Pharmacol. Exp. Ther., October 1, 2008; 327(1): 277 - 286. [Abstract] [Full Text] [PDF]

				S. Trapp, M. Isabel Aller, W. Wisden, and A. V. Gourine A Role for TASK-1 (KCNK3) Channels in the Chemosensory Control of Breathing J. Neurosci., August 27, 2008; 28(35): 8844 - 8850. [Abstract] [Full Text] [PDF]

				P. G. Guyenet The 2008 Carl Ludwig Lecture: retrotrapezoid nucleus, CO2 homeostasis, and breathing automaticity J Appl Physiol, August 1, 2008; 105(2): 404 - 416. [Abstract] [Full Text] [PDF]

				A. Cohen, Y. Ben-Abu, S. Hen, and N. Zilberberg A Novel Mechanism for Human K2P2.1 Channel Gating: FACILITATION OF C-TYPE GATING BY PROTONATION OF EXTRACELLULAR HISTIDINE RESIDUES J. Biol. Chem., July 11, 2008; 283(28): 19448 - 19455. [Abstract] [Full Text] [PDF]

				A. C. Takakura, T. S. Moreira, R. L. Stornetta, G. H. West, J. M. Gwilt, and P. G. Guyenet Selective lesion of retrotrapezoid Phox2b-expressing neurons raises the apnoeic threshold in rats J. Physiol., June 15, 2008; 586(12): 2975 - 2991. [Abstract] [Full Text] [PDF]

				P. G. Guyenet, R. L. Stornetta, and D. A. Bayliss Retrotrapezoid nucleus and central chemoreception J. Physiol., April 15, 2008; 586(8): 2043 - 2048. [Abstract] [Full Text] [PDF]

				L. A. Davies, C. Hu, N. A. Guagliardo, N. Sen, X. Chen, E. M. Talley, R. M. Carey, D. A. Bayliss, and P. Q. Barrett TASK channel deletion in mice causes primary hyperaldosteronism PNAS, February 12, 2008; 105(6): 2203 - 2208. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help