 |
The Journal of Neuroscience, December 19, 2007, 27(51):14199-14204; doi:10.1523/JNEUROSCI.4934-07.2007
Previous Article | Next Article 
Brief Communications
Gβ5 Is Required for Normal Light Responses and Morphology of Retinal ON-Bipolar Cells
Anjali Rao,1
Rebecca Dallman,1
Scott Henderson,2 and
Ching-Kang Chen1
1Departments of Biochemistry and Molecular Biology and 2Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, Virginia 23298
Correspondence should be addressed to Dr. Ching-Kang Jason Chen, Department of Biochemistry and Molecular Biology, Box 980614, Virginia Commonwealth University, 1101 E. Marshall Street, Room 2–032b, Richmond, VA 23298. Email: cjchen{at}vcu.edu
Gβ5 exists as two splice variants, Gβ5-S and Gβ5-L, which interact with and stabilize the R7 members of the regulators of G-protein signaling (RGSs): RGS6, RGS7, RGS9, and RGS11. Although the role of Gβ5-L and RGS9-1 is established in photoreceptors, the physiological functions of Gβ5-S and other R7 RGS proteins remain unclear. We found that the electroretinogram of Gβ5–/– mice lacks the b-wave component and that Gβ5-S and RGS11 colocalize with Go at the tips of the ON-bipolar cell dendrites. Unexpectedly, we found a significant reduction in the number of synaptic triads in the outer plexiform layer (OPL) of the Gβ5–/– mice, which is evident at postnatal day 14. Transgenic expression of Gβ5-L in rods failed to rescue the b-wave or the OPL defects. These results indicate that Gβ5-S is indispensable for OPL integrity and normal light responses of the retina.
Key words: bipolar cells; RGS; retina; Gβ5; morphology; ERG
Received Jan. 31, 2007;
revised Nov. 15, 2007;
accepted Nov. 15, 2007.
Correspondence should be addressed to Dr. Ching-Kang Jason Chen, Department of Biochemistry and Molecular Biology, Box 980614, Virginia Commonwealth University, 1101 E. Marshall Street, Room 2–032b, Richmond, VA 23298. Email: cjchen{at}vcu.edu
This article has been cited by other articles:

|
 |

|
 |
 
D. K. Mojumder, Y. Qian, and T. G. Wensel
Two R7 Regulator of G-Protein Signaling Proteins Shape Retinal Bipolar Cell Signaling
J. Neurosci.,
June 17, 2009;
29(24):
7753 - 7765.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. R. Anderson, R. Lujan, and K. A. Martemyanov
Changes in Striatal Signaling Induce Remodeling of RGS Complexes Containing G{beta}5 and R7BP Subunits
Mol. Cell. Biol.,
June 1, 2009;
29(11):
3033 - 3044.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Nakajima, M. Moriyama, M. Hattori, N. Minato, and S. Nakanishi
Isolation of ON Bipolar Cell Genes via hrGFP-coupled Cell Enrichment Using the mGluR6 Promoter
J. Biochem.,
June 1, 2009;
145(6):
811 - 818.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Cao, H. Song, H. Okawa, A. P. Sampath, M. Sokolov, and K. A. Martemyanov
Targeting of RGS7/G{beta}5 to the Dendritic Tips of ON-Bipolar Cells Is Independent of Its Association with Membrane Anchor R7BP
J. Neurosci.,
October 8, 2008;
28(41):
10443 - 10449.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. A. McCall and R. G. Gregg
Comparisons of structural and functional abnormalities in mouse b-wave mutants
J. Physiol.,
September 15, 2008;
586(18):
4385 - 4392.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Xu, P. Sulaiman, R. M. Feddersen, J. Liu, R. G. Smith, and N. Vardi
Retinal ON Bipolar Cells Express a New PCP2 Splice Variant That Accelerates the Light Response
J. Neurosci.,
September 3, 2008;
28(36):
8873 - 8884.
[Abstract]
[Full Text]
[PDF]
|
 |
|
|