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The Journal of Neuroscience, March 12, 2008, 28(11):2820-2826; doi:10.1523/JNEUROSCI.4145-07.2008

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Cellular/Molecular
Golgi Cell Dendrites Are Restricted by Purkinje Cell Stripe Boundaries in the Adult Mouse Cerebellar Cortex

Roy V. Sillitoe,¹ Seung-Hyuk Chung,¹ Jean-Marc Fritschy,² Monica Hoy,¹ and Richard Hawkes¹

¹Department of Cell Biology and Anatomy, Genes and Development Research Group, and Hotchkiss Brain Institute, Faculty of Medicine, The University of Calgary, Calgary, Alberta, Canada T2N 4N1, and ²Institute of Pharmacology and Toxicology, University of Zurich, CH-8057 Zurich, Switzerland

Correspondence should be addressed to Dr. Richard Hawkes, Department of Cell Biology and Anatomy, Faculty of Medicine, University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta, Canada T2N 4N1. Email: rhawkes{at}ucalgary.ca

Despite the general uniformity in cellular composition of the adult cerebellar cortex, there is a complex underlying pattern of parasagittal stripes of Purkinje cells with characteristic molecular phenotypes and patterns of connectivity. It is not known whether interneuron processes are restricted at stripe boundaries. To begin to address the issue, three strategies were used to explore how cerebellar Golgi cell dendrites are organized with respect to parasagittal stripes: first, double immunofluorescence staining combining anti-neurogranin to identify Golgi cell dendrites with the Purkinje cell compartmentation antigens zebrin II/aldolase C, HNK-1, and phospholipase Cβ4; second, zebrin II immunohistochemistry combined with a rapid Golgi–Cox impregnation procedure to reveal Golgi cell dendritic arbors; third, stripe antigen expression was used on sections of a GlyT2-EGFP transgenic mouse in which reporter expression is prominent in Golgi cell dendrites. In each case, the dendritic projections of Golgi cells were studied in the vicinity of Purkinje cell stripe boundaries. The data presented here show that the dendrites of a cerebellar interneuron, the Golgi cell, respect the fundamental cerebellar stripe cytoarchitecture.

Key words: zebrin II; HNK-1; neurogranin; compartmentation; glycine transporter 2-EGFP transgene; dendrites

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Received June 7, 2007; revised Feb. 4, 2008; accepted Feb. 4, 2008.

Correspondence should be addressed to Dr. Richard Hawkes, Department of Cell Biology and Anatomy, Faculty of Medicine, University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta, Canada T2N 4N1. Email: rhawkes{at}ucalgary.ca

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				M. E. Brown and M. Ariel Topography and Response Timing of Intact Cerebellum Stained With Absorbance Voltage-Sensitive Dye J Neurophysiol, January 1, 2009; 101(1): 474 - 490. [Abstract] [Full Text] [PDF]

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