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The Journal of Neuroscience, April 2, 2008, 28(14):3790-3803; doi:10.1523/JNEUROSCI.5658-07.2008

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Cellular/Molecular
Artificial Synaptic Conductances Reduce Subthreshold Oscillations and Periodic Firing in Stellate Cells of the Entorhinal Cortex

Fernando R. Fernandez and John A. White

Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02115

Correspondence should be addressed to Fernando R. Fernandez, Department of Bioengineering, University of Utah, 108 BPRB, 20 South 2030 East, Salt Lake City, UT 84112. Email: f.fernandez{at}utah.edu

Previous work has established that stellate cells of the medial entorhinal cortex produce prominent intrinsic subthreshold oscillations in the voltage response concentrated within the theta range (3–7 Hz). It has been speculated that these oscillations play an important role in vivo in establishing network behavior both in the entorhinal cortex and hippocampus. Consequently, it is important to investigate under what conditions theta oscillations in stellate cells can be generated and whether the spike-train power spectral density (PSD) also carries power at theta. We investigated the ability of stellate cells to generate theta oscillations in the presence of generic in vivo-like patterns of stimulation. Inputs were Poisson process-driven excitatory and inhibitory synaptic conductances or currents, introduced via dynamic clamp. We analyzed the subthreshold membrane oscillations and spike-train behavior in the presence of comparable synaptic conductance- or current-mediated membrane fluctuations. In the presence of conductance-based synapses, subthreshold oscillations are highly attenuated or entirely eliminated. Conversely, with current-based synapses stellate cells retain their ability to generate subthreshold oscillations in the theta band. These results also extend into the spiking regime, where only under current-based synapses does the PSD of the spike train show a prominent peak at theta. Furthermore, the peak in the spike-train PSD and spike clustering results from an increased probability of firing after a spike afterhyperpolarization and not directly from subthreshold oscillatory dynamics as has been previously suggested. Our results suggest that subthreshold oscillations may contribute less to in vivo response properties than has been hypothesized.

Key words: membrane conductance; medial entorhinal cortex; theta; oscillations; dynamic clamp; AHP

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Received Dec. 20, 2007; revised Feb. 5, 2008; accepted March 1, 2008.

Correspondence should be addressed to Fernando R. Fernandez, Department of Bioengineering, University of Utah, 108 BPRB, 20 South 2030 East, Salt Lake City, UT 84112. Email: f.fernandez{at}utah.edu

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