QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, April 23, 2008, 28(17):4521-4527; doi:10.1523/JNEUROSCI.5382-07.2008

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Xu, J. Articles by Disteche, C. M. Search for Related Content PubMed PubMed Citation Articles by Xu, J. Articles by Disteche, C. M.

 Previous Article  |  Next Article 

Behavioral/Systems/Cognitive
Sex-Specific Differences in Expression of Histone Demethylases Utx and Uty in Mouse Brain and Neurons

Jun Xu,^1,2,3 Xinxian Deng,¹ Rebecca Watkins,³ and Christine M. Disteche¹

¹Department of Pathology and Medicine, University of Washington, Seattle, Washington 98195, ²Department of Biomedical Sciences, Cummings School of Veterinary Medicine at Tufts University, North Grafton, Massachusetts 01536, and ³Department of Physiological Science, University of California, Los Angeles, California 90095

Correspondence should be addressed to Jun Xu, Department of Biomedical Sciences, Cummings School of Veterinary Medicine at Tufts University, North Grafton, MA 01536. Email: jun.xu{at}tufts.edu

Although X inactivation is thought to balance gene expression between the sexes, some genes escape inactivation, potentially contributing to differences between males and females. Utx (ubiquitously transcribed tetratricopeptide repeat gene on X chromosome) is an escapee gene that encodes a demethylase specific for lysine 27 of histone H3, a mark of repressed chromatin. We found Utx to be expressed higher in females than in males in developing and adult brains and in adult liver. XX mice had a higher level of Utx than XY mice, regardless of whether they had testes or ovaries, indicating that the sexually dimorphic gene expression was a consequence of the sex chromosome complement. Females had significantly higher levels of Utx than males in most brain regions except in the amygdala. The regional expression of the Y-linked paralogue Uty (ubiquitously transcribed tetratricopeptide repeat gene on Y chromosome) was somewhat distinct from that of Utx, specifically in the paraventricular nucleus of the hypothalamus (high Uty) and the amygdala (high Utx), implying that the two paralogues may be differentially regulated. Higher expression of Utx compared with Uty was detected in P19 pluripotent embryonic carcinoma cells as well as in P19-derived neurons. This transcriptional divergence between the two paralogues was associated with high levels of histone H3 lysine 4 dimethylation at the Utx promoter and of histone H4 lysine 16 acetylation throughout the gene body, which suggests that epigenetic mechanisms control differential expression of paralogous genes.

Key words: neuron; cognition; Turner syndrome; Klinefelter's syndrome; estrogen; androgen

---

Received Dec. 5, 2007; revised March 18, 2008; accepted March 18, 2008.

Correspondence should be addressed to Jun Xu, Department of Biomedical Sciences, Cummings School of Veterinary Medicine at Tufts University, North Grafton, MA 01536. Email: jun.xu{at}tufts.edu

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help