QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, June 18, 2008, 28(25):6285-6294; doi:10.1523/JNEUROSCI.0078-08.2008

This Article Full Text Full Text (PDF) Supplemental Data Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Related articles in J. Neurosci. Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Di Meglio, T. Articles by Chédotal, A. Search for Related Content PubMed PubMed Citation Articles by Di Meglio, T. Articles by Chédotal, A. Pubmed/NCBI databases Gene GEO Profiles HomoloGene Protein UniGene Substance via MeSH

 Previous Article  |  Next Article 

Development/Plasticity/Repair
Molecular Mechanisms Controlling Midline Crossing by Precerebellar Neurons

Thomas Di Meglio,^1,2 Kim T. Nguyen-Ba-Charvet,^1,2 Marc Tessier-Lavigne,³ Constantino Sotelo,^1,2,4 and Alain Chédotal^1,2

¹Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 7102, ²Université Pierre et Marie Curie, UMR 7102, F-75005 Paris, France, ³Genentech, South San Francisco, California 94080, and ⁴Cátedra de Neurobiología del Desarrollo "Remedios Caro Almela," Instituto de Neurociencias de Alicante, Universidad Miguel Hernández de Elche–Consejo Superior de Investigaciones Científicas, 03550 San Juan de Alicante, Alicante, Spain

Correspondence should be addressed to Dr. Alain Chédotal, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche en Santé 592, Institut de la Vision, F-75012 Paris, France. Email: alain.chedotal{at}inserm.fr

Precerebellar neurons of the inferior olive (IO) and lateral reticular nucleus (LRN) migrate tangentially from the rhombic lip toward the floor plate following parallel pathways. This process is thought to involve netrin-1 attraction. However, whereas the cell bodies of LRN neurons cross the midline, IO neurons are unable to do so. In many systems and species, axon guidance and cell migration at the midline are controlled by Slits and their receptor Robos. We showed previously that precerebellar axons and neurons do not cross the midline in the absence of the Robo3 receptor. To determine whether this signaling by Slits and the two other Robo receptors, Robo1 and Robo2, also regulates precerebellar neuron behavior at the floor plate, we studied the phenotype of Slit1/2 and Robo1/2/3 compound mutants. Our results showed that many IO neurons can cross the midline in absence of Slit1/2 or Robo1/2, supporting a role for midline repellents in guiding precerebellar neurons. We also show that these molecules control the development of the lamellation of the inferior olivary complex. Last, the analysis of Robo1/2/3 triple mutants suggests that Robo3 inhibits Robo1/2 repulsion in precrossing LRN axons but not in IO axons in which it has a dominant and distinct function.

Key words: cerebellum; inferior olive; floor plate; Roundabout; Slit; migration

---

Received Jan. 8, 2008; revised April 18, 2008; accepted May 7, 2008.

Correspondence should be addressed to Dr. Alain Chédotal, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche en Santé 592, Institut de la Vision, F-75012 Paris, France. Email: alain.chedotal{at}inserm.fr

Related articles in J. Neurosci.:

This Week in The Journal

J. Neurosci. 2008 28: i. [Full Text]  

This article has been cited by other articles:

				Y. Zhu, T. Matsumoto, S. Mikami, T. Nagasawa, and F. Murakami SDF1/CXCR4 signalling regulates two distinct processes of precerebellar neuronal migration and its depletion leads to abnormal pontine nuclei formation Development, June 1, 2009; 136(11): 1919 - 1928. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help