WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience MBF Stereo Investigator
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
The Journal of Neuroscience, January 16, 2008, 28(3):766-775; doi:10.1523/JNEUROSCI.3531-07.2008

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Related articles in J. Neurosci.
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via ISI Web of Science (1)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Takeuchi, H.
Right arrow Articles by Kurahashi, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Takeuchi, H.
Right arrow Articles by Kurahashi, T.

 Previous Article  |  Next Article 

Cellular/Molecular
Distribution, Amplification, and Summation of Cyclic Nucleotide Sensitivities within Single Olfactory Sensory Cilia

Hiroko Takeuchi and Takashi Kurahashi

Graduate School of Frontier Biosciences, Osaka University, Toyonaka, Osaka 560-8531, Japan

Correspondence should be addressed to Hiroko Takeuchi, Graduate School of Frontier Biosciences, Osaka University, Toyonaka, Osaka 560-8531, Japan. Email: hiroko{at}bpe.es.osaka-u.ac.jp

Submicron local cAMP elevation was used to map the distribution of transduction channels in single olfactory cilia. After the fine fluorescent visualization of the cilium with the laser-scanning confocal microscope, the intraciliary cAMP was jumped locally with the laser beam that photolyzes cytoplasmic caged compounds. Simultaneously, cells' responses were obtained with the whole-cell patch clamp. Responses were observed anywhere within the cilia, showing the broad distribution of transduction channels. For odor detection, such distribution would be useful for expanding the available responding area to increase the quantum efficiency. Also, the stimulus onto only 1 µm region induced >100 pA response operated by >700 ~2300 channels, although only 1 pA is sufficient for olfactory cells to generate action potentials. The large local response indicates a presence of strong amplification achieved with a high-density distribution of the transduction channels for the local ciliary excitation.

Key words: signal transduction; channel; cAMP; calcium; olfaction; vision

Received Aug. 3, 2007; revised Nov. 28, 2007; accepted Dec. 3, 2007.

Correspondence should be addressed to Hiroko Takeuchi, Graduate School of Frontier Biosciences, Osaka University, Toyonaka, Osaka 560-8531, Japan. Email: hiroko{at}bpe.es.osaka-u.ac.jp


Related articles in J. Neurosci.:

This Week in The Journal

J. Neurosci. 2008 28: i. [Full Text]  





-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-