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The Journal of Neuroscience, August 13, 2008, 28(33):8217-8229; doi:10.1523/JNEUROSCI.2490-08.2008

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Cellular/Molecular
Distinct Deep Short-Axon Cell Subtypes of the Main Olfactory Bulb Provide Novel Intrabulbar and Extrabulbar GABAergic Connections

Mark D. Eyre, Miklos Antal, and Zoltan Nusser

Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, 1083 Budapest, Hungary

Correspondence should be addressed to Zoltan Nusser, Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony Street 43, 1083 Budapest, Hungary. Email: nusser{at}koki.hu

A universal feature of neuronal microcircuits is the presence of GABAergic interneurons that control the activity of glutamatergic principal cells and each other. In the rat main olfactory bulb (MOB), GABAergic granule and periglomerular cells innervate mitral and tufted cells, but the source of their own inhibition remains elusive. Here, we used a combined electrophysiological and morphological approach to investigate a rather mysterious cell population of the MOB. Deep short-axon cells (dSACs) of the inframitral layers are GABAergic and have extensive and characteristic axonal ramifications in various layers of the bulb, based on which unsupervised cluster analysis revealed three distinct subtypes. Each dSAC subtype exhibits different electrical properties but receives similar GABAergic and glutamatergic inputs. The local axon terminals of all dSAC subtypes selectively innervate GABAergic granule and periglomerular cells and evoke GABA_A receptor-mediated IPSCs. One subpopulation of dSACs (GL-dSACs) creates a novel intrabulbar projection from deep to superficial layers. Another subpopulation (GCL-dSACs) is labeled by retrogradely transported fluorescent microspheres injected into higher olfactory areas, constituting a novel projection-cell population of the MOB. Our results reveal multiple dSAC subtypes, each specialized to influence MOB activity by selectively innervating GABAergic interneurons, and provide direct evidence for novel intrabulbar and extrabulbar GABAergic projections.

Key words: olfaction; inhibition; connectivity; immunohistochemistry; patch clamp; interneuron

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Received June 3, 2008; accepted June 23, 2008.

Correspondence should be addressed to Zoltan Nusser, Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony Street 43, 1083 Budapest, Hungary. Email: nusser{at}koki.hu

This article has been cited by other articles:

				A. S. Ghatpande and A. Gelperin Presynaptic Muscarinic Receptors Enhance Glutamate Release at the Mitral/Tufted to Granule Cell Dendrodendritic Synapse in the Rat Main Olfactory Bulb J Neurophysiol, April 1, 2009; 101(4): 2052 - 2061. [Abstract] [Full Text] [PDF]

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