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The Journal of Neuroscience, October 1, 2008, 28(40):10124-10133; doi:10.1523/JNEUROSCI.3769-08.2008
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Development/Plasticity/Repair
Control of Precerebellar Neuron Development by Olig3 bHLH Transcription Factor
Zijing Liu,1 Hong Li,1 Xuemei Hu,1 Ling Yu,2 Hongbin Liu,2 Ruifa Han,3 Rita Colella,1 George D. Mower,1 Yiping Chen,2 andMengsheng Qiu1,3 1Department of Anatomical Sciences and Neurobiology, University of Louisville School of Medicine, Louisville, Kentucky 40202, 2Department of Cell and Molecular Biology, Tulane University, New Orleans, Louisiana 70117, and 3Institute of Urological Surgery, Tianjin Medical University, Tianjin 300211, China
Correspondence should be addressed to Mengsheng Qiu at the above addresses. Email: m0qiu001{at}louisville.edu
The rhombic lip (RL) is the neuroepithelium immediately adjacent to the roof plate of the fourth ventricle, and it gives rise to various brainstem and cerebellar cell types. Our study shows that the bHLH (basic helix-loop-helix) transcription factor Olig3 is expressed in the progenitors of RL, and ablation of Olig3 significantly affects the development of RL. In Olig3–/– caudal RL, the expression level of Math1 in the dorsal interneuron 1 (dI1) domain is reduced, and the formation of four mossy-fiber nuclei is compromised; dI2–dI3 neurons are misspecified to dI4 interneurons, and the climbing-fiber neurons (inferior olive nucleus) are completely lost. In addition, the formation of brainstem (nor)adrenergic centers and first-order relay visceral sensory neurons is also dependent on Olig3. Therefore, Olig3 plays an important role in the fate specification and differentiation of caudal RL-derived neurons.
Key words: bHLH; cell fate; rhombic lip; mossy-fiber nuclei; climbing-fiber nucleus; cerebellum
Received Aug. 10, 2008; accepted Aug. 30, 2008.
Correspondence should be addressed to Mengsheng Qiu at the above addresses. Email: m0qiu001{at}louisville.edu
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