WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
The Journal of Neuroscience, December 3, 2008, 28(49):13216-13222; doi:10.1523/JNEUROSCI.2947-08.2008

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Petrov, A. M.
Right arrow Articles by Zefirov, A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Petrov, A. M.
Right arrow Articles by Zefirov, A. L.

 Previous Article  |  Next Article 

Development/Plasticity/Repair
The Role of cGMP-Dependent Signaling Pathway in Synaptic Vesicle Cycle at the Frog Motor Nerve Terminals

Aleksej M. Petrov,1 Arthur R. Giniatullin,1 Guzel F. Sitdikova,2 and Andrej L. Zefirov1

1Department of Normal Physiology, Kazan State Medical University, 420012 Kazan, Russia, and 2Department of Human and Animals Physiology, Kazan State University, 420008 Kazan, Russia

Correspondence should be addressed to Aleksej M. Petrov, Department of Normal Physiology, Kazan State Medical University, 49 Butlerova Street, 420012 Kazan, Russia. Email: fysio{at}rambler.ru

The role of cGMP-dependent pathways in synaptic vesicle recycling in motor nerve endings during prolonged high-frequency stimulation was studied at frog neuromuscular junctions using electrophysiological and fluorescent methods. An increase of intracellular cGMP concentration (8-Br-cGMP or 8-pCPT-cGMP) significantly reduced the cycle time for synaptic vesicles through the enhancement of vesicular traffic rate from the recycling pool to the readily releasable pool and acceleration of fast endocytosis. Pharmacological inhibition of soluble guanylate cyclase or protein kinase G slowed down the rate of recycling as well as endocytosis of synaptic vesicles. The results suggest that cGMP-PKG-dependent pathway serves a significant function in the control of vesicular cycle in frog motor terminals.

Key words: exocytosis; endocytosis; recycling of synaptic vesicles; cGMP; FM1-43; end-plate currents

Received June 26, 2008; revised Oct. 10, 2008; accepted Oct. 15, 2008.

Correspondence should be addressed to Aleksej M. Petrov, Department of Normal Physiology, Kazan State Medical University, 49 Butlerova Street, 420012 Kazan, Russia. Email: fysio{at}rambler.ru






 -
-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2009 by Society for Neuroscience ONLINE ISSN: 1529-2401
-