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The Journal of Neuroscience, December 3, 2008, 28(49):13216-13222; doi:10.1523/JNEUROSCI.2947-08.2008

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Development/Plasticity/Repair
The Role of cGMP-Dependent Signaling Pathway in Synaptic Vesicle Cycle at the Frog Motor Nerve Terminals

Aleksej M. Petrov,1 Arthur R. Giniatullin,1 Guzel F. Sitdikova,2 and Andrej L. Zefirov1

1Department of Normal Physiology, Kazan State Medical University, 420012 Kazan, Russia, and 2Department of Human and Animals Physiology, Kazan State University, 420008 Kazan, Russia

Correspondence should be addressed to Aleksej M. Petrov, Department of Normal Physiology, Kazan State Medical University, 49 Butlerova Street, 420012 Kazan, Russia. Email: fysio{at}rambler.ru

The role of cGMP-dependent pathways in synaptic vesicle recycling in motor nerve endings during prolonged high-frequency stimulation was studied at frog neuromuscular junctions using electrophysiological and fluorescent methods. An increase of intracellular cGMP concentration (8-Br-cGMP or 8-pCPT-cGMP) significantly reduced the cycle time for synaptic vesicles through the enhancement of vesicular traffic rate from the recycling pool to the readily releasable pool and acceleration of fast endocytosis. Pharmacological inhibition of soluble guanylate cyclase or protein kinase G slowed down the rate of recycling as well as endocytosis of synaptic vesicles. The results suggest that cGMP-PKG-dependent pathway serves a significant function in the control of vesicular cycle in frog motor terminals.

Key words: exocytosis; endocytosis; recycling of synaptic vesicles; cGMP; FM1-43; end-plate currents

Received June 26, 2008; revised Oct. 10, 2008; accepted Oct. 15, 2008.

Correspondence should be addressed to Aleksej M. Petrov, Department of Normal Physiology, Kazan State Medical University, 49 Butlerova Street, 420012 Kazan, Russia. Email: fysio{at}rambler.ru






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