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The Journal of Neuroscience, December 10, 2008, 28(50):13368-13383; doi:10.1523/JNEUROSCI.2918-08.2008

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Development/Plasticity/Repair
Brain-Derived Neurotrophic Factor Signaling Does Not Stimulate Subventricular Zone Neurogenesis in Adult Mice and Rats

Rui P. Galvão,^1,2 José Manuel Garcia-Verdugo,³ and Arturo Alvarez-Buylla¹

¹Department of Neurological Surgery and Institute for Regeneration Medicine, University of California at San Francisco, San Francisco, California 94143, ²Instituto Gulbenkian de Ciência, 2780 Oeiras, Portugal, and ³Laboratorio de Morfologia Celular, Unidad Mixta Centro de Investigación Príncipe Felipe, University of Valencia, El Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas, Valencia 46012, Spain

Correspondence should be addressed to Arturo Alvarez-Buylla, University of California at San Francisco, Box 0525, San Francisco, CA 94143. Email: abuylla{at}stemcell.ucsf.edu

In rodents, the adult subventricular zone (SVZ) generates neuroblasts which migrate to the olfactory bulb (OB) and differentiate into interneurons. Recent work suggests that the neurotrophin Brain-Derived Neurotrophic Factor (BDNF) can enhance adult SVZ neurogenesis, but the mechanism by which it acts is unknown. Here, we analyzed the role of BDNF and its receptor TrkB in adult SVZ neurogenesis. We found that TrkB is the most prominent neurotrophin receptor in the mouse SVZ, but only the truncated, kinase-negative isoform (TrkB-TR) was detected. TrkB-TR is expressed in SVZ astrocytes and ependymal cells, but not in neuroblasts. TrkB mutants have reduced SVZ proliferation and survival and fewer new OB neurons. To test whether this effect is cell-autonomous, we grafted SVZ cells from TrkB knock-out mice (TrkB-KO) into the SVZ of wild-type mice (WT). Grafted progenitors generated neuroblasts that migrated to the OB in the absence of TrkB. The survival and differentiation of granular interneurons and Calbindin⁺ periglomerular interneurons seemed unaffected by the loss of TrkB, whereas dopaminergic periglomerular neurons were reduced. Intra-ventricular infusion of BDNF yielded different results depending on the animal species, having no effect on neuron production from mouse SVZ, while decreasing it in rats. Interestingly, mice and rats also differ in their expression of the neurotrophin receptor p75. Our results indicate that TrkB is not essential for adult SVZ neurogenesis and do not support the current view that delivering BDNF to the SVZ can enhance adult neurogenesis.

Key words: neurotrophin; BDNF; TrkB; subventricular zone; neurogenesis; olfactory bulb; p75

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Received June 23, 2008; revised Sept. 20, 2008; accepted Oct. 11, 2008.

Correspondence should be addressed to Arturo Alvarez-Buylla, University of California at San Francisco, Box 0525, San Francisco, CA 94143. Email: abuylla{at}stemcell.ucsf.edu

This article has been cited by other articles:

				M. Snapyan, M. Lemasson, M. S. Brill, M. Blais, M. Massouh, J. Ninkovic, C. Gravel, F. Berthod, M. Gotz, P. A. Barker, et al. Vasculature Guides Migrating Neuronal Precursors in the Adult Mammalian Forebrain via Brain-Derived Neurotrophic Factor Signaling J. Neurosci., April 1, 2009; 29(13): 4172 - 4188. [Abstract] [Full Text] [PDF]

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