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The Journal of Neuroscience, April 8, 2009, 29(14):4664-4674; doi:10.1523/JNEUROSCI.5502-08.2009

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Behavioral/Systems/Cognitive
Activity Profiles of Cholinergic and Intermingled GABAergic and Putative Glutamatergic Neurons in the Pontomesencephalic Tegmentum of Urethane-Anesthetized Rats

Soufiane Boucetta and Barbara E. Jones

Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada H3A 2B4

Correspondence should be addressed to Dr. Barbara E. Jones, Montreal Neurological Institute, McGill University, 3801 University Street, Montreal, Quebec, Canada H3A 2B4. Email: barbara.jones{at}mcgill.ca

Cholinergic neurons in the pontomesencephalic tegmentum form part of the ascending activating system and are thought to participate in stimulating cortical activation. Yet in the laterodorsal tegmental and pedunculopontine tegmental nuclei (LDT and PPT), they lie intermingled with GABAergic and glutamatergic neurons, which could also modulate cortical activity and sleep–wake state. To characterize the discharge of these cell types in relation to cortical activity, we recorded neurons in urethane-anesthetized rats during spontaneous slow wave and somatosensory evoked fast electroencephalographic (EEG) activity, then labeled the cells by juxtacellular technique with Neurobiotin (Nb) and dual-immunostained them for vesicular acetylcholine transporter (VAChT) and glutamic acid decarboxylase (GAD). All cholinergic cells discharged minimally during prestimulation (0.5 Hz) and moderately in a tonic manner (4 Hz) during stimulation. Being heterogeneous, some GABAergic, called "On," cells (48%) increased their discharge (from 4 to 7 Hz), whereas others, called "Off" cells (38%), decreased or ceased firing during stimulation. Similarly, some noncholinergic/non-GABAergic On cells increased (from 2 to 6 Hz, 49%), whereas other Off cells decreased firing (35%) during stimulation. Putative glutamatergic On together with GABAergic On neurons could thus act in parallel with cholinergic cells to stimulate cortical activation. Possibly influenced by cholinergic On and glutamatergic Off cells, whose change in discharge precedes theirs, the GABAergic Off cells could oppose neighboring neurons such as noradrenergic cells, which discharge during waking and cease firing during sleep. By concerted activity, these heterogeneous cell groups can modulate cortical activity and behavioral state across the sleep–waking cycle.

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Received Nov. 14, 2008; revised March 9, 2009; accepted March 12, 2009.

Correspondence should be addressed to Dr. Barbara E. Jones, Montreal Neurological Institute, McGill University, 3801 University Street, Montreal, Quebec, Canada H3A 2B4. Email: barbara.jones{at}mcgill.ca

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