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The Journal of Neuroscience, April 29, 2009, 29(17):5558-5572; doi:10.1523/JNEUROSCI.0520-09.2009

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Cellular/Molecular
The Translational Repressors Nanos and Pumilio Have Divergent Effects on Presynaptic Terminal Growth and Postsynaptic Glutamate Receptor Subunit Composition

Kaushiki P. Menon,¹ Shane Andrews,² Mala Murthy,¹ Elizabeth R. Gavis,² and Kai Zinn¹

¹Division of Biology, California Institute of Technology, Pasadena, California 91125, and ²Department of Molecular Biology, Princeton University, Princeton, New Jersey 09544

Correspondence should be addressed to either Kaushiki P. Menon or Kai Zinn, Division of Biology 114-96, California Institute of Technology, Pasadena, CA 91125. Email: menonk{at}caltech.edu or Email: zinnk{at}caltech.edu

Pumilio (Pum) is a translational repressor that binds selectively to target mRNAs and recruits Nanos (Nos) as a corepressor. In the larval neuromuscular system, Pum represses expression of the translation factor eIF-4E and the glutamate receptor subunit GluRIIA. Here, we show that Nos, like Pum, is expressed at the neuromuscular junction (NMJ) and in neuronal cell bodies. Surprisingly, however, Nos and Pum have divergent functions on both the presynaptic and postsynaptic sides of the NMJ. In nos mutant and nos RNA interference larvae, the number of NMJ boutons is increased, whereas loss of Pum reduces the bouton number. On the postsynaptic side, Nos acts in opposition to Pum in regulating the subunit composition of the glutamate receptor. NMJ active zones are associated with GluRIIA- and GluRIIB-containing receptor clusters. Loss of Nos causes downregulation of GluRIIA and increases the levels of GluRIIB. Consistent with this finding, the electrophysiological properties of NMJs lacking postsynaptic Nos suggest that they use primarily GluRIIB-containing receptors. Nos can regulate GluRIIB in the absence of GluRIIA, suggesting that the effects of Nos on GluRIIB levels are at least partially independent of synaptic competition between GluRIIA and GluRIIB. Nos is a target for Pum repression, and Pum binds selectively to the 3' untranslated regions of the nos and GluRIIA mRNAs. Our results suggest a model in which regulatory interplay among Pum, Nos, GluRIIA, and GluRIIB could cause a small change in Pum activity to be amplified into a large shift in the balance between GluRIIA and GluRIIB synapses.

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Received Jan. 31, 2009; revised Feb. 27, 2009; accepted March 20, 2009.

Correspondence should be addressed to either Kaushiki P. Menon or Kai Zinn, Division of Biology 114-96, California Institute of Technology, Pasadena, CA 91125. Email: menonk{at}caltech.edu or Email: zinnk{at}caltech.edu

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