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The Journal of Neuroscience, June 24, 2009, 29(25):8248-8258; doi:10.1523/JNEUROSCI.5287-08.2009

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Cellular/Molecular
NOS1AP Regulates Dendrite Patterning of Hippocampal Neurons through a Carboxypeptidase E-Mediated Pathway

Damien Carrel,^1 * Yangzhou Du,^1 * Daniel Komlos,^1,2 Norell M. Hadzimichalis,¹ Munjin Kwon,^1,3 Bo Wang,¹ Linda M. Brzustowicz,⁴ and Bonnie L. Firestein¹

¹Department of Cell Biology and Neuroscience, ²Neuroscience Graduate Program, ³Molecular Biosciences Graduate Program, and ⁴Department of Genetics, Rutgers University, Piscataway, New Jersey 08854-8082

Correspondence should be addressed to Dr. Bonnie L. Firestein, Department of Cell Biology and Neuroscience, Rutgers University, 604 Allison Road, Piscataway, NJ 08854-8082. Email: firestein{at}biology.rutgers.edu

During neuronal development, neurons form elaborate dendritic arbors that receive signals from axons. Additional studies are needed to elucidate the factors regulating the establishment of dendritic patterns. Our work explored possible roles played by nitric oxide synthase 1 adaptor protein (NOS1AP; also known as C-terminal PDZ ligand of neuronal nitric oxide synthase or CAPON) in dendritic patterning of cultured hippocampal neurons. Here we report that the long isoform of NOS1AP (NOS1AP-L) plays a novel role in regulating dendrite outgrowth and branching. NOS1AP-L decreases dendrite number when overexpressed at any interval between day in vitro (DIV) 0 and DIV 12, and knockdown of NOS1AP-L results in increased dendrite number. In contrast, the short isoform of NOS1AP (NOS1AP-S) decreases dendrite number only when overexpressed during DIV 5–7. Using mutants of NOS1AP-L, we show that neither the PDZ-binding domain nor the PTB domain is necessary for the effects of NOS1AP-L. We have functionally narrowed the region of NOS1AP-L that mediates this effect to the middle amino acids 181–307, a region that is not present in NOS1AP-S. Furthermore, we performed a yeast two-hybrid screen and identified carboxypeptidase E (CPE) as a binding partner for the middle region of NOS1AP-L. Biochemical and cellular studies reveal that CPE mediates the effects of NOS1AP on dendrite morphology. Together, our results suggest that NOS1AP-L plays an important role in the initiation, outgrowth, and maintenance of dendrites during development.

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Received Nov. 3, 2008; revised May 8, 2009; accepted May 26, 2009.

Correspondence should be addressed to Dr. Bonnie L. Firestein, Department of Cell Biology and Neuroscience, Rutgers University, 604 Allison Road, Piscataway, NJ 08854-8082. Email: firestein{at}biology.rutgers.edu

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