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The Journal of Neuroscience, July 1, 2009, 29(26):8438-8446; doi:10.1523/JNEUROSCI.1284-09.2009

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Cellular/Molecular
ASIC2 Subunits Target Acid-Sensing Ion Channels to the Synapse via an Association with PSD-95

Xiang-ming Zha,1,3 Vivian Costa,2,3 Anne Marie S. Harding,3 Leah Reznikov,1,3 Christopher J. Benson,2,3 and Michael J. Welsh1,2,3,4

1Howard Hughes Medical Institute, 2Neuroscience Program, and 3Departments of Internal Medicine and 4Molecular Physiology and Biophysics, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242

Correspondence should be addressed to either of the following: Michael J. Welsh, Howard Hughes Medical Institute, Roy J. & Lucille A. Carver College of Medicine, Iowa City, IA 52242, Email: michael-welsh{at}uiowa.edu; or Christopher J. Benson, Roy J. & Lucille A. Carver College, Department of Internal Medicine, E314-1 GH, Iowa City, IA 52242-1009, E-mail: Email: chris-benson{at}uiowa.edu

Acid-sensing ion channel-1a (ASIC1a) mediates H+-gated current to influence normal brain physiology and impact several models of disease. Although ASIC2 subunits are widely expressed in brain and modulate ASIC1a current, their function remains poorly understood. We identified ASIC2a in dendrites, dendritic spines, and brain synaptosomes. This localization largely relied on ASIC2a binding to PSD-95 and matched that of ASIC1a, which does not coimmunoprecipitate with PSD-95. We found that ASIC2 and ASIC1a associated in brain, and through its interaction with PSD-95, ASIC2 increased ASIC1a localization in dendritic spines. Consistent with earlier work showing that acidic pH elevated spine [Ca2+]i by activating ASIC1a, loss of ASIC2 decreased the percentage of spines responding to acid. Moreover, like a reduction of ASIC1a, the number of spine synapses fell in ASIC2–/– neurons. These results indicate that ASIC2 facilitates ASIC1a localization and function in dendritic spines and suggest that the two subunits work in concert to regulate neuronal function.

Received March 16, 2009; revised April 24, 2009; accepted May 13, 2009.

Correspondence should be addressed to either of the following: Michael J. Welsh, Howard Hughes Medical Institute, Roy J. & Lucille A. Carver College of Medicine, Iowa City, IA 52242, Email: michael-welsh{at}uiowa.edu; or Christopher J. Benson, Roy J. & Lucille A. Carver College, Department of Internal Medicine, E314-1 GH, Iowa City, IA 52242-1009, E-mail: Email: chris-benson{at}uiowa.edu






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