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The Journal of Neuroscience, July 22, 2009, 29(29):9292-9300; doi:10.1523/JNEUROSCI.6063-08.2009

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Behavioral/Systems/Cognitive
Proteinase-Activated Receptors in the Nucleus of the Solitary Tract: Evidence for Glial–Neural Interactions in Autonomic Control of the Stomach

Gerlinda E. Hermann,¹ Montina J. Van Meter,¹ Jennifer C. Rood,² and Richard C. Rogers¹

¹Autonomic Neuroscience Laboratory and ²Clinical Chemistry Core, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, Louisiana 70808

Correspondence should be addressed to Dr. Gerlinda Hermann, Laboratory of Autonomic Neuroscience, Pennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, LA 70808. Email: gerlinda.hermann{at}pbrc.edu

Bleeding head injury is associated with gastric stasis, a symptom of collapse of autonomic control of the gut described by Cushing around 1932. Recent work suggests that the proteinase thrombin, produced secondary to bleeding, may be the root cause. Results from our in vivo physiological studies show that fourth ventricular injection of PAR1 agonists, as well as thrombin itself, produced significant reductions in gastric transit in the awake rat. We expected that the PAR1 effect to inhibit gastric transit was the result of direct action on vagovagal reflex circuitry in the dorsal medulla. Surprisingly, our immunohistochemical studies demonstrated that PAR1 receptors are localized exclusively to the astrocytes and not the neurons in the nucleus of the solitary tract (NST; principal locus integrating visceral afferent input and part of the gastric vagovagal reflex control circuitry). Our in vitro calcium imaging studies of hindbrain slices revealed that PAR1 activation initially causes a dramatic increase in astrocytic calcium, followed seconds later by an increase in calcium signal in NST neurons. The neuronal effect, but not the astrocytic effect, of PAR1 activation was eliminated by glutamate receptor antagonism. TTX did not eliminate the effects of PAR1 activation on either glia or neurons. Thus, we propose that glia are the primary CNS sensors for PAR agonists and that the response of these glial cells drives the activity of adjacent (e.g., NST) neurons. These results show, for the first time, that changes in autonomic control can be directly signaled by glial detection of local chemical stimuli.

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Received Dec. 19, 2008; revised May 18, 2009; accepted June 17, 2009.

Correspondence should be addressed to Dr. Gerlinda Hermann, Laboratory of Autonomic Neuroscience, Pennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, LA 70808. Email: gerlinda.hermann{at}pbrc.edu

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