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The Journal of Neuroscience, January 21, 2009, 29(3):863-870; doi:10.1523/JNEUROSCI.2818-08.2009

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Cellular/Molecular
F-Actin and Myosin II Accelerate Catecholamine Release from Chromaffin Granules

Khajak Berberian,1,2 * Alexis J. Torres,3 * Qinghua Fang,1 Kassandra Kisler,1 and Manfred Lindau1

1School of Applied and Engineering Physics, and Departments of 2Biomedical Engineering and 3Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853

Correspondence should be addressed to Dr. Manfred Lindau, School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853. Email: ml95{at}cornell.edu

The roles of nonmuscle myosin II and cortical actin filaments in chromaffin granule exocytosis were studied by confocal fluorescence microscopy, amperometry, and cell-attached capacitance measurements. Fluorescence imaging indicated decreased mobility of granules near the plasma membrane following inhibition of myosin II function with blebbistatin. Slower fusion pore expansion rates and longer fusion pore lifetimes were observed after inhibition of actin polymerization using cytochalasin D. Amperometric recordings revealed increased amperometric spike half-widths without change in quantal size after either myosin II inhibition or actin disruption. These results suggest that actin and myosin II facilitate release from individual chromaffin granules by accelerating dissociation of catecholamines from the intragranular matrix possibly through generation of mechanical forces.

Key words: chromaffin cells; exocytosis; actin; myosin II; amperometry; capacitance; fluorescence microscopy; fusion pore

Received June 19, 2008; revised Nov. 28, 2008; accepted Dec. 15, 2008.

Correspondence should be addressed to Dr. Manfred Lindau, School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853. Email: ml95{at}cornell.edu




This article has been cited by other articles:


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 Mol. Biol. CellHome page
B. W. Doreian, T. G. Fulop, R. L. Meklemburg, and C. B. Smith
Cortical F-Actin, the Exocytic Mode, and Neuropeptide Release in Mouse Chromaffin Cells Is Regulated by Myristoylated Alanine-rich C-Kinase Substrate and Myosin II
Mol. Biol. Cell, July 1, 2009; 20(13): 3142 - 3154.
[Abstract] [Full Text] [PDF]


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