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The Journal of Neuroscience, July 29, 2009, 29(30):9405-9416; doi:10.1523/JNEUROSCI.2356-09.2009

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Development/Plasticity/Repair
Tenascin-C Is an Inhibitory Boundary Molecule in the Developing Olfactory Bulb

Helen B. Treloar,¹ Arundhati Ray,¹ Lu Anne Dinglasan,¹ Melitta Schachner,^3,4 and Charles A. Greer^1,2

Departments of ¹Neurosurgery and ²Neurobiology, Yale University School of Medicine, New Haven, Connecticut 06520-8082, ³Zentrum für Molekulare Neurobiologie, Universitätskrankenhaus Hamburg-Eppendorf, D-20246 Hamburg, Germany, and ⁴Keck Center for Collaborative Neuroscience and Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, New Jersey 08901

Correspondence should be addressed to Dr. Helen B. Treloar, Department of Neurosurgery, Yale University School of Medicine, P.O. Box 208082, 333 Cedar Street, New Haven, CT 06520-8082. Email: helen.treloar{at}yale.edu

We recently described the boundary-like expression pattern of the extracellular matrix molecule tenascin-C (Tnc) in the developing mouse olfactory bulb (OB) (Shay et al., 2008). In the present study, we test the hypothesis that Tnc inhibits olfactory sensory neuron (OSN) axon growth in the developing OB before glomerulogenesis. The period of time before glomerular formation begins, when axons remain restricted to the developing olfactory nerve layer (ONL), is crucial for axon sorting. Here, we show with in vitro analyses that OSN neurite outgrowth is inhibited by Tnc in a dose-dependent manner and that, in stripe assays, axons preferentially avoid Tnc. Using Tnc-null mice, we also show that that glomerular development is delayed in the absence of Tnc. In wild-type mice, OSN axons coalesce into immature or protoglomeruli, which further differentiate and segregate into glomeruli. Glomeruli are first identifiable as discrete structures at birth. In null mice, glomeruli appear immature at birth, remain fused to the ONL, and have a significantly larger diameter compared with wild-type controls. By postnatal day 4, null glomeruli are indistinguishable from controls. Thus, OSN axons appear delayed in their coalescence into glomerular structures. These data correlate with behavioral reports of Tnc-null mice, which are delayed by 24 h in their acquisition of an olfactory behavior (de Chevigny et al., 2006). Collectively, these data demonstrate that Tnc is an inhibitory boundary molecule in the developing OB during a key period of development.

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Received May 19, 2009; accepted June 23, 2009.

Correspondence should be addressed to Dr. Helen B. Treloar, Department of Neurosurgery, Yale University School of Medicine, P.O. Box 208082, 333 Cedar Street, New Haven, CT 06520-8082. Email: helen.treloar{at}yale.edu

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