The Journal of Neuroscience, July 29, 2009, 29(30):9592-9601; doi:10.1523/JNEUROSCI.2162-09.2009
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Cellular/Molecular
Excitability Changes Related to GABAA Receptor Plasticity during Pregnancy
Jamie Maguire,1
Isabella Ferando,1
Charlotte Simonsen,2 and
Istvan Mody1
1Departments of Neurology and Physiology, The David Geffen School of Medicine, University of California, Los Angeles, California 90095, and 2Department of Pharmacology and Pharmacotherapy, Faculty of Pharmaceutical Sciences, University of Copenhagen, DK-1165 Copenhagen, Denmark
Correspondence should be addressed to Dr. Istvan Mody, Department of Neurology, NRB1 Room 575D, The David Geffen School of Medicine, University of California, Los Angeles, 635 Charles Young Drive South, Los Angeles, CA 90095-7332. Email: mody{at}ucla.edu
Alterations in GABAA receptor (GABAAR) expression and function, similar to those we described previously during pregnancy in the mouse dentate gyrus, may also occur in other brain regions. Here we show, using immunohistochemical techniques, a decreased 
subunit-containing GABAAR (GABAAR) expression in the dentate gyrus, hippocampal CA1 region, thalamus, and striatum but not in the cerebral cortex. In the face of the highly elevated neurosteroid levels during pregnancy, which can act on GABAARs, it may be beneficial to decrease the number of neurosteroid-sensitive receptors to maintain a steady-state level of neuronal excitability throughout pregnancy. Consistent with this hypothesis, the synaptic input/output (I/O) relationship in the dentate gyrus molecular layer in response to lateral perforant path stimulation was shifted to the left in hippocampal slices from pregnant compared with virgin mice. The addition of allopregnanolone, at levels comparable with those found during pregnancy (100 nM), shifted the I/O curves in pregnant mice back to virgin levels. There was a decreased threshold to induce epileptiform local field potentials in slices from pregnant mice compared with virgin, but allopregnanolone reverted the threshold for inducing epileptiform activity to virgin levels. According to these data, neuronal excitability is increased in pregnant mice in the absence of allopregnanolone attributable to brain region-specific downregulation of GABAAR expression. In brain regions, such as the cortex, that do not exhibit alterations in GABAAR expression, there were no changes in the I/O relationship during pregnancy. Similarly, no changes in network excitability were detected in pregnant Gabrd–/– mice that lack GABAARs, suggesting that changes in neuronal excitability during pregnancy are attributable to alterations in the expression of these receptors. Our findings indicate that alterations in GABAAR expression during pregnancy result in brain region-specific increases in neuronal excitability that are restored by the high levels of allopregnanolone under normal conditions but under pathological conditions may result in neurological and psychiatric disorders associated with pregnancy and postpartum.
Received May 7, 2009;
revised June 14, 2009;
accepted June 19, 2009.
Correspondence should be addressed to Dr. Istvan Mody, Department of Neurology, NRB1 Room 575D, The David Geffen School of Medicine, University of California, Los Angeles, 635 Charles Young Drive South, Los Angeles, CA 90095-7332. Email: mody{at}ucla.edu
