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The Journal of Neuroscience, August 26, 2009, 29(34):10488-10498; doi:10.1523/JNEUROSCI.2355-09.2009

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Development/Plasticity/Repair
Negative Guidance Factor-Induced Macropinocytosis in the Growth Cone Plays a Critical Role in Repulsive Axon Turning

Adrianne L. Kolpak,^1 * Jun Jiang,^1 * Daorong Guo,¹ Clive Standley,² Karl Bellve,² Kevin Fogarty,² and Zheng-Zheng Bao¹

¹Department of Medicine and Cell Biology, Program in Neuroscience, and ²Biomedical Imaging Group, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605

Correspondence should be addressed to Zheng-Zheng Bao, Department of Medicine and Cell Biology, Program in Neuroscience, University of Massachusetts Medical School, Worcester, MA 01605. Email: zheng.bao{at}umassmed.edu

Macropinocytosis is a type of poorly characterized fluid-phase endocytosis that results in formation of relatively large vesicles. We report that Sonic hedgehog (Shh) protein induces macropinocytosis in the axons through activation of a noncanonical signaling pathway, including Rho GTPase and nonmuscle myosin II. Macropinocytosis induced by Shh is independent of clathrin-mediated endocytosis but dependent on dynamin, myosin II, and Rho GTPase activities. Inhibitors of macropinocytosis also abolished the negative effects of Shh on axonal growth, including growth cone collapse and chemorepulsive axon turning but not turning per se. Conversely, activation of myosin II or treatment of phorbol ester induces macropinocytosis in the axons and elicits growth cone collapse and repulsive axon turning. Furthermore, macropinocytosis is also induced by ephrin-A2, and inhibition of dynamin abolished repulsive axon turning induced by ephrin-A2. Macropinocytosis can be induced ex vivo by high Shh, correlating with axon retraction. These results demonstrate that macropinocytosis-mediated membrane trafficking is an important cellular mechanism involved in axon chemorepulsion induced by negative guidance factors.

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Received May 19, 2009; revised July 3, 2009; accepted July 15, 2009.

Correspondence should be addressed to Zheng-Zheng Bao, Department of Medicine and Cell Biology, Program in Neuroscience, University of Massachusetts Medical School, Worcester, MA 01605. Email: zheng.bao{at}umassmed.edu

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