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The Journal of Neuroscience, September 16, 2009, 29(37):11674-11685; doi:10.1523/JNEUROSCI.1542-09.2009

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Development/Plasticity/Repair
Axonal Targeting of Trk Receptors via Transcytosis Regulates Sensitivity to Neurotrophin Responses

Maria Ascaño, Alissa Richmond, Philip Borden, and Rejji Kuruvilla

Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218

Correspondence should be addressed to Rejji Kuruvilla, Department of Biology, Johns Hopkins University, Baltimore, MD 21218. Email: rkuruvilla{at}jhu.edu

Axonal targeting of trophic receptors is critical for neuronal responses to extracellular developmental cues, yet the underlying trafficking mechanisms remain unclear. Here, we report that tropomyosin-related kinase (Trk) receptors for target-derived neurotrophins are anterogradely trafficked to axons via transcytosis in sympathetic neurons. Using compartmentalized cultures, we show that mature receptors on neuronal soma surfaces are endocytosed and remobilized via Rab11-positive recycling endosomes into axons. Inhibition of dynamin-dependent endocytosis disrupted anterograde transport and localization of TrkA receptors in axons. Anterograde TrkA delivery and exocytosis into axon growth cones is enhanced by nerve growth factor (NGF), acting locally on distal axons. Perturbing endocytic recycling attenuated NGF-dependent signaling and axon growth while enhancing recycling conferred increased neuronal sensitivity to NGF. Our results reveal regulated transcytosis as an unexpected mode of Trk trafficking that serves to rapidly mobilize ready-synthesized receptors to growth cones, thus providing a positive feedback mechanism by which limiting concentrations of target-derived neurotrophins enhance neuronal sensitivity.

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Received March 31, 2009; revised July 31, 2009; accepted Aug. 11, 2009.

Correspondence should be addressed to Rejji Kuruvilla, Department of Biology, Johns Hopkins University, Baltimore, MD 21218. Email: rkuruvilla{at}jhu.edu

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