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The Journal of Neuroscience, October 14, 2009, 29(41):12940-12947; doi:10.1523/JNEUROSCI.3525-09.2009

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Cellular/Molecular
Positive and Negative Regulation of Odor Receptor Gene Choice in Drosophila by Acj6

Lei Bai, Aaron L. Goldman, and John R. Carlson

Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520

Correspondence should be addressed to Dr. John R. Carlson, Department of Molecular, Cellular, and Developmental Biology, Yale University, P.O. Box 208103, New Haven, CT 06520-8103. Email: john.carlson{at}yale.edu

Little is known about how individual olfactory receptor neurons (ORNs) select, from among many odor receptor genes, which genes to express. Abnormal chemosensory jump 6 (Acj6) is a POU domain transcription factor essential for the specification of ORN identity and odor receptor (Or) gene expression in the Drosophila maxillary palp, one of the two adult olfactory organs. However, the mechanism by which Acj6 functions in this process has not been investigated. Here, we systematically examine the role of Acj6 in the maxillary palp and in a major subset of antennal ORNs. We define an Acj6 binding site by a reiterative in vitro selection process. The site is found upstream of Or genes regulated by Acj6, and Acj6 binds to the site in Or promoters. Mutational analysis shows that the site is essential for Or regulation in vivo. Surprisingly, a novel ORN class in acj6 adults is found to arise from ectopic expression of a larval Or gene, which is repressed in wild type via an Acj6 binding site. Thus, Acj6 acts directly in the process of receptor gene choice; it plays a dual role, positive and negative, in the logic of the process, and acts in partitioning the larval and adult receptor repertoires.

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Received July 21, 2009; revised Aug. 27, 2009; accepted Sept. 2, 2009.

Correspondence should be addressed to Dr. John R. Carlson, Department of Molecular, Cellular, and Developmental Biology, Yale University, P.O. Box 208103, New Haven, CT 06520-8103. Email: john.carlson{at}yale.edu

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