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The Journal of Neuroscience, November 4, 2009, 29(44):13909-13918; doi:10.1523/JNEUROSCI.2351-09.2009

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Cellular/Molecular
Autocrine and Paracrine Roles for ATP and Serotonin in Mouse Taste Buds

Yijen A. Huang,¹ Robin Dando,¹ and Stephen D. Roper^1,2

¹Department of Physiology and Biophysics and ²Program in Neuroscience, University of Miami School of Medicine, Miami, Florida 33136

Correspondence should be addressed to Dr. Stephen D. Roper, Department of Physiology and Biophysics, University of Miami School of Medicine, 1600 Northwest 10th Avenue, Miami, FL 33136. Email: roper{at}miami.edu

Receptor (type II) taste bud cells secrete ATP during taste stimulation. In turn, ATP activates adjacent presynaptic (type III) cells to release serotonin (5-hydroxytryptamine, or 5-HT) and norepinephrine (NE). The roles of these neurotransmitters in taste buds have not been fully elucidated. Here we tested whether ATP or 5-HT exert feedback onto receptor (type II) cells during taste stimulation. Our previous studies showed NE does not appear to act on adjacent taste bud cells, or at least on receptor cells. Our data show that 5-HT released from presynaptic (type III) cells provides negative paracrine feedback onto receptor cells by activating 5-HT_1A receptors, inhibiting taste-evoked Ca²⁺ mobilization in receptor cells, and reducing ATP secretion. The findings also demonstrate that ATP exerts positive autocrine feedback onto receptor (type II) cells by activating P2Y1 receptors and enhancing ATP secretion. These results begin to sort out how purinergic and aminergic transmitters function within the taste bud to modulate gustatory signaling in these peripheral sensory organs.

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Received May 19, 2009; revised Sept. 22, 2009; accepted Sept. 24, 2009.

Correspondence should be addressed to Dr. Stephen D. Roper, Department of Physiology and Biophysics, University of Miami School of Medicine, 1600 Northwest 10th Avenue, Miami, FL 33136. Email: roper{at}miami.edu

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