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The Journal of Neuroscience, November 11, 2009, 29(45):14199-14210; doi:10.1523/JNEUROSCI.2873-09.2009

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Cellular/Molecular
Overexpressing Temperature-Sensitive Dynamin Decelerates Phototransduction and Bundles Microtubules in Drosophila Photoreceptors

Paloma T. Gonzalez-Bellido,¹ Trevor J. Wardill,¹ Ripsik Kostyleva,² Ian A. Meinertzhagen,² and Mikko Juusola^1,3

¹Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, United Kingdom, ²Department of Psychology and Neuroscience, Dalhousie University, Halifax, Nova Scotia 3BH 4J1, Canada, and ³State Key Laboratory of Cognitive Neuroscience, Beijing Normal University, Beijing 100875, China

Correspondence should be addressed to either of the following: Mikko Juusola, Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, UK; Email: m.juusola{at}sheffield.ac.uk; or Ian A. Meinertzhagen, Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS 3BH 4J1, Canada, Email: iam{at}dal.ca

shibire^ts1, a temperature-sensitive mutation of the Drosophila gene encoding a Dynamin orthologue, blocks vesicle endocytosis and thus synaptic transmission, at elevated, or restrictive temperatures. By targeted Gal4 expression, UAS-shibire^ts1 has been used to dissect neuronal circuits. We investigated the effects of UAS-shibire^ts1 overexpression in Drosophila photoreceptors at permissive (19°C) and restrictive (31°C) temperatures. At 19°C, overexpression of UAS-shi^ts1 causes decelerated phototransduction and reduced neurotransmitter release. This phenotype is exacerbated with dark adaptation, age and in white mutants. Photoreceptors overexpressing UAS-shibire^ts1 contain terminals with widespread vacuolated mitochondria, reduced numbers of vesicles and bundled microtubules. Immuno-electron microscopy reveals that the latter are dynamin coated. Further, the microtubule phenotype is not restricted to photoreceptors, as UAS-shibire^ts1 overexpression in lamina cells also bundles microtubules. We conclude that dynamin has multiple functions that are interrupted by UAS-shibire^ts1 overexpression in Drosophila photoreceptors, destabilizing their neural communication irreversibly at previously reported permissive temperatures.

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Received June 17, 2009; revised Aug. 28, 2009; accepted Sept. 22, 2009.

Correspondence should be addressed to either of the following: Mikko Juusola, Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, UK; Email: m.juusola{at}sheffield.ac.uk; or Ian A. Meinertzhagen, Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS 3BH 4J1, Canada, Email: iam{at}dal.ca

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