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The Journal of Neuroscience, December 2, 2009, 29(48):15245-15257; doi:10.1523/JNEUROSCI.3606-09.2009

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Cellular/Molecular
Interneurons Produced in Adulthood Are Required for the Normal Functioning of the Olfactory Bulb Network and for the Execution of Selected Olfactory Behaviors

Vincent Breton-Provencher,¹ Morgane Lemasson,¹ Modesto R. Peralta III,¹ and Armen Saghatelyan^1,2

¹Cellular Neurobiology Unit, Centre de Recherche Université Laval Robert-Giffard, Québec, Québec G1J 2G3, Canada, and ²Departement of Psychiatry, Université Laval, Québec, Québec G1K 7P4, Canada

Correspondence should be addressed to Armen Saghatelyan, Centre de Recherche Université Laval Robert-Giffard, Université Laval, 2601 chemin de la Canardiere, Québec, QC G1J 2G3, Canada. Email: armen.saghatelyan{at}crulrg.ulaval.ca

Olfactory bulb (OB) interneurons are continuously renewed throughout an animal's lifespan. Despite extensive investigation of this phenomenon, little is known about bulbar circuitry functioning and olfactory performances under conditions of ablated arrival of new neurons into the adult OB. To address this issue we performed morphological, electrophysiological, and behavioral analysis in mice with suppressed bulbar neurogenesis. Infusion of the antimitotic drug AraC to the lateral ventricle via 28 d osmotic minipumps abolished the arrival of newly born neurons into the adult OB without affecting the total number of granule cells. The number, dendritic arborization, and spine density of interneurons generated in adulthood, before pump installation, were also not affected by AraC treatment. As a result of ablated neurogenesis, mitral cells—the principal output neurons in the OB—receive fewer inhibitory synapses, display reduced frequency of spontaneous IPSCs, experience smaller dendrodendritic inhibition, and exhibit decreased synchronized activity. Consequently, short-term olfactory memory was drastically reduced in AraC-treated mice. In contrast, olfactory performances of AraC-treated animals were undistinguishable from those of control mice in other odor-associated tests, such as spontaneous odor discrimination and long-term odor-associative memory tasks. Altogether, our data highlight the importance of adult neurogenesis for the proper functioning of the OB network and imply that new bulbar interneurons are involved in some, but not all, odor-associated tasks.

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Received July 25, 2009; revised Sept. 24, 2009; accepted Oct. 26, 2009.

Correspondence should be addressed to Armen Saghatelyan, Centre de Recherche Université Laval Robert-Giffard, Université Laval, 2601 chemin de la Canardiere, Québec, QC G1J 2G3, Canada. Email: armen.saghatelyan{at}crulrg.ulaval.ca

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