 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 3, 2251-2262, Copyright © 1983 by Society for Neuroscience
Optical monitoring of activity from many areas of the in vitro and in vivo salamander olfactory bulb: a new method for studying functional organization in the vertebrate central nervous system
HS Orbach and LB Cohen
We have investigated the use of voltage-sensitive dyes to monitor neuronal
activity in the intact salamander olfactory bulb. After a 10- to 20-min
staining period, a magnified image of an in vitro or an in vivo preparation
was formed on a 124-element photodiode array. The array was used to
simultaneously record absorption or fluorescence changes from 124 adjacent
areas of the bulb. At the magnifications used, each detector received light
from 100 to 1000 neurons. Relatively large absorption and fluorescence
signals were found in response to olfactory nerve stimulation; all of the
results presented were from single trials. Because of the large signal
size, measurements on in vivo preparations using epi-illumination also had
good signal-to-noise ratios. There were significant differences in signal
time course between adjacent detectors which suggested a spatial resolution
on the order of 200 microns. Tentative assignments of the cellular origins
of some signals could be made from the results of paired volley
experiments. The results suggest that optical monitoring of membrane
potential could provide a useful method for studying neuronal organization
in the intact vertebrate central nervous system.
This article has been cited by other articles:
|
|
|
|
 
M. Fukuda, U. M. Rajagopalan, R. Homma, M. Matsumoto, M. Nishizaki, and M. Tanifuji
Localization of Activity-dependent Changes in Blood Volume to Submillimeter-scale Functional Domains in Cat Visual Cortex
Cereb Cortex,
June 1, 2005;
15(6):
823 - 833.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Polimeni, D. Granquist-Fraser, R. J. Wood, and E. L. Schwartz
Physical limits to spatial resolution of optical recording: Clarifying the spatial structure of cortical hypercolumns
PNAS,
March 15, 2005;
102(11):
4158 - 4163.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Momose-Sato, Y. Honda, H. Sasaki, and K. Sato
Optical Mapping of the Functional Organization of the Rat Trigeminal Nucleus: Initial Expression and Spatiotemporal Dynamics of Sensory Information Transfer during Embryogenesis
J. Neurosci.,
February 11, 2004;
24(6):
1366 - 1376.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Shoham and A. Grinvald
The Cortical Representation of the Hand in Macaque and Human Area S-I: High Resolution Optical Imaging
J. Neurosci.,
September 1, 2001;
21(17):
6820 - 6835.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. C. Carlson, M. T. Shipley, and A. Keller
Long-Lasting Depolarizations in Mitral Cells of the Rat Olfactory Bulb
J. Neurosci.,
March 1, 2000;
20(5):
2011 - 2021.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-W. Lam, L. B. Cohen, M. Wachowiak, and M. R. Zochowski
Odors Elicit Three Different Oscillations in the Turtle Olfactory Bulb
J. Neurosci.,
January 15, 2000;
20(2):
749 - 762.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. ANTIC, L. B. COHEN, Y.-W. LAM, M. WACHOWIAK, D. ZECEVIC, and M. ZOCHOWSKI
Fast multisite optical measurement of membrane potential: three examples
FASEB J,
December 1, 1999;
13(9002):
271S - 276S.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Wachowiak and L. B. Cohen
Presynaptic Inhibition of Primary Olfactory Afferents Mediated by Different Mechanisms in Lobster and Turtle
J. Neurosci.,
October 15, 1999;
19(20):
8808 - 8817.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Yagodin, C. Collin, D. L. Alkon, N. F. Sheppard Jr., and D. B. Sattelle
Mapping Membrane Potential Transients in Crayfish (Procambarus clarkii) Optic Lobe Neuropils With Voltage-Sensitive Dyes
J Neurophysiol,
January 1, 1999;
81(1):
334 - 344.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. P. Bindokas, C. C. Lee, W. F. Colmers, and R. J. Miller
Changes in Mitochondrial Function Resulting from Synaptic Activity in the Rat Hippocampal Slice
J. Neurosci.,
June 15, 1998;
18(12):
4570 - 4587.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Keller, S. Yagodin, V. Aroniadou-Anderjaska, L. A. Zimmer, M. Ennis, N. F. Sheppard Jr, and M. T. Shipley
Functional Organization of Rat Olfactory Bulb Glomeruli Revealed by Optical Imaging
J. Neurosci.,
April 1, 1998;
18(7):
2602 - 2612.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Prechtl, L. B. Cohen, B. Pesaran, P. P. Mitra, and D. Kleinfeld
Visual stimuli induce waves of electrical activity in turtle cortex
PNAS,
July 8, 1997;
94(14):
7621 - 7626.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
