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Journal of Neuroscience, Vol 3, 2403-2413, Copyright © 1983 by Society for Neuroscience
Neurite regeneration by Aplysia neurons in dissociated cell culture: modulation by Aplysia hemolymph and the presence of the initial axonal segment
S Schacher and E Proshansky
Neurons from the abdominal ganglion of the mollusc Aplysia californica
regenerate neurite processes in dissociated cell culture. Both the nature
of neurite outgrowth and the morphology of the cells are influenced by the
presence of adult Aplysia hemolymph in the growth medium and the presence
of a portion of a cell's original axonal process. Aplysia hemolymph
enhances cell survival, the initiation of neurite outgrowth from multiple
sites on the cell body surface, the linear growth of the processes, and the
amount of branching by those processes. Hemolymph also decreases the
diameter of the outgrowing neurite fascicles and the diameter of the
individual neurites within the fascicles. The presence of a cell's original
axon reduces the time required for the initiation of neurite outgrowth and
restricts the formation of multipolar processes. In addition, the presence
of an initial axonal segment is essential for neurite regeneration from
large adult neurons.
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B Edmonds, M Klein, N Dale, and E. Kandel
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J. Levenson, S. Endo, L. S. Kategaya, R. I. Fernandez, D. G. Brabham, J. Chin, J. H. Byrne, and A. Eskin
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PNAS,
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