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Journal of Neuroscience, Vol 3, 1694-1700, Copyright © 1983 by Society for Neuroscience
Slowing of the axonal transport of neurofilament proteins during development
PN Hoffman, RJ Lasek, JW Griffin and DL Price
We examined age-dependent changes in neurofilament transport in motor axons
of the rat sciatic nerve. SDS-PAGE and gel fluorography confirmed that the
distribution of labeled neurofilament triplet protein coincides with the
major slow component a (SCa) wave in these neurons. The velocity of
neurofilament transport was calculated on the basis of the location of the
50th percentile Of radioactivity in this wave 33 days after motor neurons
were labeled by the intraspinal administration of [3H]leucine and
[3H]lysine. Overall, the velocity fell from 1.95 mm/day at 3 weeks of age
to 1.12 mm/day at 20 weeks. Between 3 and 10 weeks, it fell at a 6-fold
higher rate (0.096 mm/day/week) than between 10 and 20 weeks (0.016
mm/day/week). We also found a marked change in the shape of the slow
component wave during development. It appeared to consist of several
overlapping peaks moving at slightly different velocities in animals 10
weeks of age or less as compared to a single slower moving peak at 20
weeks. We propose that the velocity of slow axonal transport reflects the
level of maturation of the neuron, and that the presence of several
overlapping peaks Of transported radioactivity in the sciatic nerve of
younger animals reflects the presence of several populations of motor axons
at different stages of development. We also discuss the relationship
between changes in the velocity of neurofilament transport and alterations
in the composition of the cytoskeleton that occur as the axon grows in
caliber during postnatal development.
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