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Journal of Neuroscience, Vol 4, 2518-2527, Copyright © 1984 by Society for Neuroscience
Pattern and specificity of axonal outgrowth following varying degrees of chick limb bud ablation
KW Tosney and LT Landmesser
Motoneurons grow into the chick hindlimb via consistent pathways, within
which they make specific decisions leading to their correct targets. To
determine which axonal guidance features dictate the position of the
pathways and to examine the distribution of specific cues, we totally or
partially ablated the early hindlimb bud and determined how the subsequent
pattern of nerve outgrowth related to the distribution of tissue remnants.
Our results suggest that local elements determine the gross anatomical
pattern of outgrowth. First, determinants of individual pathways could be
selectively removed without altering the pattern in other regions. Second,
neurites were restricted to the plexus region at the base of the leg
(within which, for unknown reasons, they proceeded posteriorly) unless
distal permissive pathways or nearby target remnants were present. Finally,
we found that the central region of the pelvic girdle, adjacent to the
plexus region, determines the position where the major nerve trunks enter
the leg. When gaps were introduced in this region of the girdle, nerves
traversed the gaps and directly innervated adjacent muscle. The developing
girdle is probably a nonpermissive environment for axon elongation, and
axons enter the leg only where it is locally absent. Our results also
support the concept that the specific cues that neurites use to reach their
appropriate muscles are local. We find that neurites could make correct and
specific decisions in the plexus region in the absence of all tissues
distal to the pelvic girdle. This shows that the cues for these decisions
are independent of the target and must reside in the local mesenchyme. In
addition, when muscle remnants were present they were correctly
innervated.(ABSTRACT TRUNCATED AT 250 WORDS)
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