Journal of Neuroscience, Vol 4, 793-802, Copyright © 1984 by Society for Neuroscience
An in vitro model of ischemia: metabolic and electrical alterations in the hippocampal slice
TS Whittingham, WD Lust and JV Passonneau
The transverse guinea pig hippocampal slice preparation was used to model
the metabolic changes which occur in vivo during ischemia and recovery.
Perfusing brain slices with medium devoid of glucose and oxygen elicits
rapid decreases in phosphocreatine, ATP, intracellular pH, and in the
evoked field potential recorded in the dentate gyrus. AMP and creatine rise
during this period, while ADP and lactate levels remain unchanged. Cyclic
AMP exhibits a transient increase in concentration. With the exception of
ADP and lactate, these responses are very similar to those observed during
in vivo ischemia. The return of glucose and oxygen to the incubation medium
reverses these metabolic and electrophysiological effects and also leads to
pronounced elevations in cyclic nucleotide concentrations. Metabolite
concentrations approach, but do not reach, in vitro steady state levels
during the first 30 min of recovery. Total adenylate and creatine steady
state levels are approximately 50% of in vivo concentrations. The results
suggest that, although hippocampal slices differ metabolically from in vivo
tissue, they exhibit a similar pattern of metabolic responses to ischemic
and reflow conditions.
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