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Journal of Neuroscience, Vol 4, 820-831, Copyright © 1984 by Society for Neuroscience
The D1.1 antigen: a cell surface marker for germinal cells of the central nervous system
JM Levine, L Beasley and WB Stallcup
A monoclonal antibody against a cell surface ganglioside present on
neuroepithelial cells was produced by immunizing mice with the B49 cell
line, a clonal line with properties of both neurons and glial cells. The
expression of this antigen, designated as D1.1, was analyzed in the
developing rat cerebellum. The D1.1 antigen was localized by the
immunofluorescent staining method to germinal cells of the external granule
layer (EGL). Fluorescent labeling of cells comprising the EGL was apparent
on embryonic day 18 when the EGL first forms, and the labeling was present
throughout the period of postnatal cerebellar development. No cells within
the adult cerebellum were labeled with the anti-D1.1 antibody. The
D1.1-labeled cells of the EGL synthesize DNA, as demonstrated by [3H]
thymidine autoradiography. However, within 48 hr after their final mitosis,
nascent cerebellar cells that had migrated away from the external granule
layer were no longer labeled with antibody. Some of the neurons and some of
the astrocytes in cerebellar cell cultures were fluorescently labeled with
the anti-D1.1 antibody. The number of anti-D1.1-labeled neurons in the
cultures decreased over the first 10 days in vitro in agreement with the
findings that in vivo the fluorescent labeling of the D1.1 antigen
disappears from postmitotic cells. The antibody recognizes a ganglioside
that in thin layer chromatographic experiments has a mobility between that
of the GM1 and GM2 ganglioside. These data suggest that the D1.1
ganglioside antigen is a cell surface marker for germinal cells and that
the acquisition and subsequent loss of this antigen is an aspect of the
biochemical maturation of neurons and glial cells.
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