 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 4, 1683-1689, Copyright © 1984 by Society for Neuroscience
Uptake of nerve growth factor along peripheral and spinal axons of primary sensory neurons
PM Richardson and RJ Riopelle
To investigate the distribution of nerve growth factor (NGF) receptors on
peripheral and central axons, [125I]NGF was injected into the sciatic nerve
or spinal cord of adult rats. Accumulation of [125I]NGF in lumbar dorsal
root ganglia was monitored by gamma emission counting and radioautography.
[125I]NGF, injected endoneurially in small quantities, was taken into
sensory axons by a saturable process and was transported retrogradely to
their cell bodies at a maximal rate of 2.5 to 7.5 mm/hr. Because very
little [125I]NGF reached peripheral terminals, the results were interpreted
to indicate that receptors for NGF are present on nonterminal segments of
sensory axons. The specificity and high affinity of NGF uptake were
illustrated by observations that negligible amounts of gamma activity
accumulated in lumbar dorsal root ganglia after comparable intraneural
injection of [125I] cytochrome C or [125I]oxidized NGF. Similar techniques
were used to demonstrate avid internalization and retrograde transport of
[125I]NGF by intraspinal axons arising from dorsal root ganglia. Following
injection of [125I]NGF into lumbar or cervical regions of the spinal cord,
neuronal perikarya were clearly labeled in radioautographs of lumbar dorsal
root ganglia. Sites for NGF uptake on primary sensory neurons in the adult
rat are not restricted to peripheral axon terminals but are extensively
distributed along both peripheral and central axons. Receptors on axons
provide a mechanism whereby NGF supplied by glia could influence neuronal
maintenance or axonal regeneration.
This article has been cited by other articles:
|
|
|
|
 
M. Melanson, P. Miao, D. Eisenstat, Y. Gong, X. Gu, K. Au, W. Zhu, F. Begum, E. Frost, and M. Namaka
Experimental autoimmune encephalomyelitis-induced upregulation of tumor necrosis factor-alpha in the dorsal root ganglia
Multiple Sclerosis,
October 1, 2009;
15(10):
1135 - 1145.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S. A. Mousa, B. P. Cheppudira, M. Shaqura, O. Fischer, J. Hofmann, R. Hellweg, and M. Schafer
Nerve growth factor governs the enhanced ability of opioids to suppress inflammatory pain
Brain,
February 1, 2007;
130(2):
502 - 513.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. L. Watson, H. M. Heerssen, D. B. Moheban, M. Z. Lin, C. M. Sauvageot, A. Bhattacharyya, S. L. Pomeroy, and R. A. Segal
Rapid Nuclear Responses to Target-Derived Neurotrophins Require Retrograde Transport of Ligand-Receptor Complex
J. Neurosci.,
September 15, 1999;
19(18):
7889 - 7900.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Bhattacharyya, F. L. Watson, T. A. Bradlee, S. L. Pomeroy, C. D. Stiles, and R. A. Segal
Trk Receptors Function As Rapid Retrograde Signal Carriers in the Adult Nervous System
J. Neurosci.,
September 15, 1997;
17(18):
7007 - 7016.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. R. Ure and R. B. Campenot
Retrograde Transport and Steady-State Distribution of 125I-Nerve Growth Factor in Rat Sympathetic Neurons in Compartmented Cultures
J. Neurosci.,
February 15, 1997;
17(4):
1282 - 1290.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
