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Journal of Neuroscience, Vol 4, 2269-2280, Copyright © 1984 by Society for Neuroscience
Transient and differential expression of aspects of the catecholaminergic phenotype during development of the fetal bowel of rats and mice
MD Gershon, TP Rothman, TH Joh and GN Teitelman
A population of proliferating cells in the fetal gut has previously been
found to express transiently aspects of a catecholaminergic phenotype (TC
cells) during development in both rats and mice. These cells appear to be
noradrenergic in rats but dopaminergic in mice. In the current study, the
ability of TC cells, identified by the immunocytochemical demonstration of
tyrosine hydroxylase (TH), to take up and become radioautographically
labeled by [3H]norepinephrine ([3H]NE) was assessed. When TC cells were
most numerous in the bowel of rats, no cells were labeled by [3H]NE (days
E12 and E13). In rats, but not mice, labeling of larger cell bodies by
[3H]NE was found on days E14 and E15. However, no cells showed TH
immunoreactivity on day E15, although a few cells were doubly labeled by
[3H]NE and TH immunoreactivity on day E14. Therefore, in rats TC cells
contain TH immunoreactivity but do not take up [3H]NE prior to day E14, and
their disappearance is followed by the appearance of a second population of
larger cells that lacks TH immunoreactivity but which does take up [3H]NE.
The transient appearance of some cells that express both markers on day E14
suggests, but does not prove, that TC cells change their phenotype and are
the precursors of the cells found later in development that lack TH but
which take up [3H]NE. The cells that take up [3H]NE are rare or absent in
newborn rat gut, indicating that they may also be transient. These results
indicate that genes responsible for different aspects of the noradrenergic
phenotype need not necessarily be coupled in their expression. Although
uptake of [3H] NE into cell bodies was not found on day E13 or later in
vivo in mouse gut, it does occur in mouse bowel explanted prior to day E13
and grown for 10 to 12 days in culture. These cultures also contained TH
immunoreactive cells. Thus, the potential for development of cells able to
take up [3H]NE exists in mice as well as in rats, and the conditions that
lead to a loss of catecholaminergic traits in vivo do not exist in vitro.
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